In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 71, No. 8_Supplement ( 2011-04-15), p. 5213-5213
Abstract:
Neck lymph node involvement is considered as the most important adverse prognostic factor in head and neck squamous cell carcinoma (HNSCC). Patients with clinical stage I/II (T1-2N0M0) oral tongue carcinoma usually undergo partial glossectomy alone. However, 14 to 48% of these patients develop delayed neck metastasis (DNM) within 2 years and tend to result in unfavorable course. Clinicopathologic features and molecular biomarkers have been investigated to find the reliable predictor of DNM, however, it still remains to be elucidated. Recently E-cadherin and its transcriptional repressors are thought to play a crucial role in tumor progression and metastasis, the mechanism of which is called epithelial to mesenchymal transition (EMT). The present study was aimed to examine whether the molecular factors involved in the induction of EMT, as well as standard clinicopathologic factors, are predictive of DNM in stage I/II oral tongue squamous cell carcinoma. mRNA expressions of E-cadherin and its repressors (snail, SIP1 and twist) in 7 HNSCC cell lines were evaluated by quantitative real-time RT-PCR, which revealed significant inverse correlation between E-cadherin and SIP1 (p = 0.023). Immunohistochemical staining was performed to evaluate protein expressions of E-cadherin and its repressors in the specimens of the primary lesions of 37 stage I/II tongue carcinoma patients who had initially undergone only partial glossectomy in the Keio University Hospital without elective neck dissection. Univariate analysis showed that loss of E-cadherin (p = 0.004) and overexpression of SIP1 (p & lt; 0.001) were significantly correlated with DNM, although no inverse correlation was found between E-cadherin and each of its repressors. Regarding other clinicopathologic factors, mode of invasion 3/4 (p = 0.048), vascular invasion (p = 0.021) and muscular invasion (p = 0,012) were also significantly correlated with DNM. Multiple logistic regression analysis including clinicopathologic and the molecular factors elucidated that overexpression of SIP1 (p = 0.005), loss of E-cadherin (p = 0.046) and vascular invasion (p = 0.024) were independently correlated with DNM. These results suggest that DNM in the stage I/II oral tongue carcinoma is closely relating to EMT, and especially SIP1 and E-cadherin are considered as the useful markers to predict development of DNM. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 102nd Annual Meeting of the American Association for Cancer Research; 2011 Apr 2-6; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2011;71(8 Suppl):Abstract nr 5213. doi:10.1158/1538-7445.AM2011-5213
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2011-5213
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2011
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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