In:
FEBS Letters, Wiley, Vol. 396, No. 2-3 ( 1996-11-04), p. 147-151
Abstract:
In plants, calcium‐dependent protein kinases (CDPKs) make up a large family that is characterized by a C‐terminal calmodulin(CaM)‐like domain. Recently, a novel carrot cDNA clone encoding an atypical CDPK, which has a significantly degenerate sequence in the CaM‐like domain, was found and named CDPK‐related protein kinase (CRK) [Lindzen, E. and Choi, J.H. (1995) Plant Mol. Biol. 28, 785–797]. We obtained two different cDNA clones from maize which encode CRKs. For the first enzymatic characterization of CRK, a maize cDNA clone was expressed in E. coli . The recombinant protein efficiently phosphorylated casein, a conventional protein substrate. Notably, in this in vitro phosphorylation assay, the kinase activity did not require calcium as an activator. Thus, CRKs were suggested to be novel calcium‐independent protein kinases having a degenerate CaM domain, the function of which remains to be elucidated.
Type of Medium:
Online Resource
ISSN:
0014-5793
,
1873-3468
DOI:
10.1016/0014-5793(96)01090-3
Language:
English
Publisher:
Wiley
Publication Date:
1996
detail.hit.zdb_id:
212746-5
detail.hit.zdb_id:
1460391-3
SSG:
12
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