In:
Science and Technology Development Journal, Viet Nam National University Ho Chi Minh City, Vol. 17, No. 2 ( 2014-06-30), p. 45-51
Abstract:
CLEA method (cross-linking enzyme aggregates) combines enzyme preparation and immobilization from solution culture into the same step. In this study, we applied CLEA method to immobilize two enzymes, glucoamylase and pectinase, from crude enzyme solution obtained from semi-solid culture of Aspergillus niger. The results showed that: In the same immobilized conditions (glucoamylase: 7% glutaraldehyde, 5°C, 2 hours; pectinase: 10% glutaraldehyde, 5°C, 2 hours), the immobilized enzyme from crude enzyme solution, has the abilities to be reused and activation stability under the influences of pH and temperature higher than immobilized commercial enzyme respectively.
Type of Medium:
Online Resource
ISSN:
1859-0128
,
1859-0128
DOI:
10.32508/stdj.v17i2.1358
Language:
Unknown
Publisher:
Viet Nam National University Ho Chi Minh City
Publication Date:
2014
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