In:
Mediators of Inflammation, Wiley, Vol. 2006, No. 1 ( 2006-01)
Abstract:
Inflammatory processes are known to be involved at least in the
early phase of complex regional pain syndrome type 1 (CRPS1). Blister fluid obtained from the involved extremities displayed
increased amounts of proinflammatory cytokines IL‐6 and TNF α compared with the noninvolved extremities. The aim of this paper
is to investigate the involvement of mediators by measurement of several other cytokines using new detection techniques that enable
multiple cytokine measurement in small samples. The use of a multiplex‐25 bead array cytokine assay and Luminex technology
enabled simultaneous measurement of representative (1) proinflammatory cytokines such as GM‐CSF, IL‐1 β , IL‐1RA,
IL‐6, IL‐8, and TNF‐ α ; (2) Th1/Th2 distinguishing
cytokines IFN‐ γ , IL‐2, IL‐2R, IL‐4, IL‐5, and IL‐10; (3)
nonspecific acting cytokines IFN‐ α , IL‐7, IL‐12p40/p70,
IL‐13, IL‐15, and IL‐17; and (4) chemokines eotaxin, IP‐10, MCP‐1, MIP‐1 α , MIP‐1 β , MIG, and RANTES. Although minimal
detection levels are significantly higher in the bead array system than those in common ELISA assays, in blister fluid, IL‐1RA, IL‐6,
IL‐8, TNF‐ α , IL‐12p40/p70, MCP‐1, and MIP‐1 β were
detectable and increased in CRPS1 affected extremities. Levels of IL‐6 and TNF‐ α simultaneously measured by ELISA (Sanquin
Compact kit) and by multiplex‐25 bead array assay (Biosource) were highly correlated ( r = 0.85, P 〈 .001
for IL‐6 and r = 0.88, P 〈 .001 for TNF‐ α ). Furthermore, IP‐10 and eotaxin were
detectable but diminished in CRPS1, whereas detectable amounts of IL‐10 were similar in involved and noninvolved extremities.
Multiplex bead array assays are useful systems to establish the involvement of cytokines in inflammatory processes by measurements
in blister fluids of CRPS1. Ten representative cytokines were detectable. However, detection levels and amounts measured are at
least 3 times higher in the multiplex‐25 array assay than in the ELISA assays used simultaneously for the measurement of cytokines.
Type of Medium:
Online Resource
ISSN:
0962-9351
,
1466-1861
DOI:
10.1155/MI/2006/28398
Language:
English
Publisher:
Wiley
Publication Date:
2006
detail.hit.zdb_id:
1137605-3
detail.hit.zdb_id:
2008065-7
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