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  • 1
    Online Resource
    Online Resource
    Streptococcus thermophilus JM905—Strain Carbon Source Utilization and Its Fermented Milk Metabolic Profile at Different Fermentation Stages
    MDPI AG ; 2023
    In:  Foods Vol. 12, No. 19 ( 2023-10-08), p. 3690-
    Details
    In: Foods, MDPI AG, Vol. 12, No. 19 ( 2023-10-08), p. 3690-
    Abstract: The metabolic utilization of different carbon sources by Streptococcus thermophilus JM905(S. thermophilus JM905) was determined using a high-throughput microbial phenotyping system, and changes in fermentation characteristics of S. thermophilus JM905 fermented milk were investigated at different fermentation periods, with changes in pH, water-holding capacity, viscosity, nuisance odor, and viable bacteria count being used to define the fermentation characteristics of the strain. Changes in the key metabolites, 2-hydroxybutyric acid, folic acid, L-lactic acid, D-glycerol-D-galactose-heptanol, (R)-leucine, L-aspartic acid, L-proline, D-arginine, L-isoleucine, hydra starch, L-lysine, L-tryptophan, and D-galactose, were clarified. Correspondingly, the fermented milk protein, amino acid, and fermented milk fat quality nutrient contents were determined to be 3.78 ± 0.054 g per 100 g, 3.405 ± 0.0234 g per 100 mL, and 0.161 ± 0.0030 g per 100 g, respectively. This study addressed strain carbon source utilization, changes in fermentation characteristics and metabolites during fermentation, with the aim of investigating the link between fermentation characteristics and metabolite quality components of Streptococcus thermophilus JM905 and its fermented milk with fermentation potential and to provide a useful reference for the screening of superior fermentation strains.
    Type of Medium: Online Resource
    ISSN: 2304-8158
    URL: Article
    DOI: 10.3390/foods12193690
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2704223-6
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  • 2
    Online Resource
    Online Resource
    Data_Sheet_1.PDF
    Frontiers Media SA ; 2023
    In:  Frontiers in Microbiology Vol. 13 ( 2023-1-6)
    Details
    In: Frontiers in Microbiology, Frontiers Media SA, Vol. 13 ( 2023-1-6)
    Abstract: Long-chain fatty acids in breast milk are affected by the mother’s diet and play an important role in the growth, development, and immune construction of infants. This study aims to explore the correlation between maternal diet, breast milk fatty acids (FAs), and the infant intestinal flora. Methods We enrolled 56 paired mothers and their infants; both breast milk samples and infants’ fecal samples were collected to determine the long-chain FA content of breast milk by ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS), and metagenomic technology was applied to determine the microbial composition of infant feces. The maternal diet was also investigated using a 24-h dietary recall. Results The results indicated that the fat contribution rates of edible oils in the maternal diet are significantly positively correlated with the contents of certain long-chain fatty acids (C16:0, C18:1, C16:1, and C22:4) in breast milk, which mainly regulate the abundance of Lacticaseibacillus rhamnosus, Lacticaseibacillus fermentum , and Lacticaseibacillus paracasei in the infant gut. Through KEGG pathway analysis, our data revealed that the long-chain FAs in different groups of breast milk were significantly correlated with the pathways of biotin metabolism, glycerolipid metabolism, and starch and sucrose metabolism. Discussion The results of this study suggest a pathway in which the diets of lactating mothers may affect the composition of the infant intestinal microbiota by influencing breast milk FAs and then further regulating infant health.
    Type of Medium: Online Resource
    ISSN: 1664-302X
    URL: Article
    URL: Article
    DOI: 10.3389/fmicb.2022.1004175
    DOI: 10.3389/fmicb.2022.1004175.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2587354-4
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  • 3
    Online Resource
    Online Resource
    Syntheses, crystal structures and non-linear optical properties of two novel windmill-shaped clusters: [M2Pd4S8(dppm)2]·4DMF (M = W or Mo)
    Royal Society of Chemistry (RSC) ; 2000
    In:  Journal of the Chemical Society, Dalton Transactions , No. 13 ( 2000), p. 2145-2149
    Details
    In: Journal of the Chemical Society, Dalton Transactions, Royal Society of Chemistry (RSC), , No. 13 ( 2000), p. 2145-2149
    Type of Medium: Online Resource
    ISSN: 1470-479X , 1364-5447
    URL: Article
    DOI: 10.1039/b000616p
    Language: Unknown
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2000
    detail.hit.zdb_id: 1472887-4
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  • 4
    Online Resource
    Online Resource
    Effect of the catabolic control protein A of Lactiplantibacillus plantarum AR113 on its colonization in vivo
    Wiley ; 2024
    In:  Food Frontiers
    Details
    In: Food Frontiers, Wiley
    Abstract: We constructed a ccpA knockout strain (AR113ΔccpA) to investigate the impact of carbohydrate metabolism on the in vivo colonization of AR113. The knockout of the ccpA gene was significantly affecting the proliferation of AR113 in an in vitro experiment. Our work demonstrated that the ccpA does not play a crucial role in the in vivo colonization time of AR113.
    Type of Medium: Online Resource
    ISSN: 2643-8429 , 2643-8429
    URL: Article
    DOI: 10.1002/fft2.378
    Language: English
    Publisher: Wiley
    Publication Date: 2024
    detail.hit.zdb_id: 3021468-3
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  • 5
    Online Resource
    Online Resource
    Effects of Coix seed extract, Lactobacillus paracasei K56, and their combination on the glycolipid metabolism in obese mice
    Wiley ; 2023
    In:  Journal of Food Science Vol. 88, No. 3 ( 2023-03), p. 1197-1213
    Details
    In: Journal of Food Science, Wiley, Vol. 88, No. 3 ( 2023-03), p. 1197-1213
    Abstract: Coix seed extract (CSE) and probiotics have been reported to regulate glycolipid metabolism through different modes of action. We tested the effects of CSE, Lactobacillus paracasei K56, and their combination to determine whether they have synergistic effects on glycolipid metabolism of obese mice. We fed male C57BL/6J mice with high‐fat diet for 8 weeks to establish an obesity model. The obesity mice were selected and divided into five groups: the model control group and four intervention groups. After 10 weeks of continuous gavage intervention, the mice in the intervention groups exhibited lower body weight (lower about 2.31–4.41 g, vs. HFD 42.25 g, p   〈  0.01), and epididymal (lower about 0.58–0.92 g, vs. HFD 2.50 g, p   〈  0.01) and perirenal fat content (lower about 0.24–0.42 g, vs. HFD 0.88 g, p   〈  0.05); decreased fasting blood glucose, total cholesterol, triglycerides, and VLDL; and increased HLDL, respiratory exchange ratio, energy expenditure, and amount of exercise performed. K56 + CSE‐combined intervention groups were more effective in lowering blood glucose, IL‐1β, and TNF‐α levels than the CSE and K56 alone interventions. The content of fatty acid synthase and SREBP‐1c protein in liver tissue was lower. The combination has synergistic effects on weight control, fat reduction, and blood glucose regulation by improving the chronic inflammatory state and reducing the content of lipid synthesis‐related enzymes of obese mice, which can hinder chronic disease progression. Practical Application Coix seed extract can be used in obese people to regulate abnormal glucose and lipid metabolism and delay the development of chronic diseases.
    Type of Medium: Online Resource
    ISSN: 0022-1147 , 1750-3841
    URL: Issue
    URL: Article
    DOI: 10.1111/jfds.v88.3
    DOI: 10.1111/1750-3841.16474
    Language: English
    Publisher: Wiley
    Publication Date: 2023
    detail.hit.zdb_id: 2006705-7
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  • 6
    Online Resource
    Online Resource
    Table_1.XLSX
    Frontiers Media SA ; 2022
    In:  Frontiers in Nutrition Vol. 9 ( 2022-7-18)
    Details
    In: Frontiers in Nutrition, Frontiers Media SA, Vol. 9 ( 2022-7-18)
    Abstract: Coix seed extract (CSE) and probiotics have been reported to regulate glycolipid metabolism via different modes of action. We tested the effects of CSE, Bifidobacterium BPL1, and their combination to determine their effects on glycolipid metabolism in obese mice. Male C57BL/6J mice were fed a high-fat diet for 8 weeks to establish an obesity model. Obese mice were selected and divided into four groups: the model control group and three intervention groups. After 10 weeks of continuous gavage intervention, the mice in the intervention groups exhibited lower body weight (lower about 2.31 g, vs. HFD mice 42.23 g) and epididymal (lower about 0.37 g, vs. HFD mice 2.5 g) and perirenal fat content (lower about 0.47 g, vs. HFD mice 0.884 g); decreased fasting blood glucose, total cholesterol, triglycerides, and VLDL; and increased HLDL, respiratory exchange ratio, energy expenditure, and amount of exercise performed. CSE, BPL1 and their combination can effectively control the weight gain in obese mice, reduce fat content, and regulate blood lipids and abnormal blood sugar. These results may be related to reduce the chronic inflammatory states, improve energy metabolism, exercise, relieve insulin sensitivity, and reduce lipid synthesis via the intervention of CSE, BPL1 and their combination. Compared with the single use of CSE alone, the combination of CSE + BPL1 can better exert the regulation function of intestinal flora, and change in the abundance of bacteria that could improve the level of inflammatory factors, such as increasing Bifidobacterium , reducing Lactococcus . Compared with the use of BPL1 alone, the combination of CSE and BPL1 can better regulate pancreatic islet and improve blood sugar. CSE may act directly on body tissues to exert anti-inflammatory effects. BPL1 and CSE + BPL1 may improve the structure and function of the intestinal flora, and reduce tissue inflammation.
    Type of Medium: Online Resource
    ISSN: 2296-861X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fnut.2022.939423
    DOI: 10.3389/fnut.2022.939423.s001
    DOI: 10.3389/fnut.2022.939423.s002
    DOI: 10.3389/fnut.2022.939423.s003
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2022
    detail.hit.zdb_id: 2776676-7
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  • 7
    Online Resource
    Online Resource
    Effect of Thermal Inactivation on Antioxidant, Anti-Inflammatory Activities and Chemical Profile of Postbiotics
    MDPI AG ; 2023
    In:  Foods Vol. 12, No. 19 ( 2023-09-26), p. 3579-
    Details
    In: Foods, MDPI AG, Vol. 12, No. 19 ( 2023-09-26), p. 3579-
    Abstract: Inactivation is a crucial step in the production of postbiotics, with thermal inactivation being the prevailing method employed. Nevertheless, the impact of thermal treatment on bioactivity and chemical composition remains unexplored. The objective of this study was to assess the influence of heating temperature on the antioxidant, anti-inflammatory properties and the chemical composition of ET-22 and BL-99 postbiotics. The findings revealed that subjecting ET-22 and BL-99 to thermal treatment ranging from 70 °C to 121 °C for a duration of 10 min effectively deactivated them, leading to the disruption of cellular structure and release of intracellular contents. The antioxidant and anti-inflammatory activity of ET-22 and BL-99 postbiotics remained unaffected by mild heating temperatures (below 100 °C). However, excessive heating at 121 °C diminished the antioxidant activity of the postbiotic. To further investigate the impact of thermal treatments on chemical composition, non-targeted metabolomics was conducted to analyze the cell-free supernatants derived from ET-22 and BL-99. The results revealed that compared to mild inactivation at temperatures below 100 °C, the excessive temperature of 121 °C significantly altered the chemical profile of the postbiotic. Several bioactive components with antioxidant and anti-inflammatory properties, including zomepirac, flumethasone, 6-hydroxyhexanoic acid, and phenyllactic acid, exhibited a significant reduction in their levels following exposure to a temperature of 121 °C. This decline in their abundance may be associated with a corresponding decrease in their antioxidant and anti-inflammatory activities. The cumulative evidence gathered strongly indicates that heating temperatures exert a discernible influence on the properties of postbiotics, whereby excessive heating leads to the degradation of heat-sensitive active constituents and subsequent diminishment of their biological efficacy.
    Type of Medium: Online Resource
    ISSN: 2304-8158
    URL: Article
    DOI: 10.3390/foods12193579
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2704223-6
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  • 8
    Online Resource
    Online Resource
    Bifidobacterium lactis BL-99 modulates intestinal inflammation and functions in zebrafish models
    Public Library of Science (PLoS) ; 2022
    In:  PLOS ONE Vol. 17, No. 2 ( 2022-2-16), p. e0262942-
    Details
    In: PLOS ONE, Public Library of Science (PLoS), Vol. 17, No. 2 ( 2022-2-16), p. e0262942-
    Abstract: This study was designed to explore the therapeutics and the mechanisms of a patented and marked gastric acid and intestine juice-resistant probiotics Bifidobacterium lactis BL-99 ( B . lactis BL-99) on the intestinal inflammation and functions in the zebrafish models. After feeding for 6 hours, B . lactis BL-99 was fully retained in the larval zebrafish intestinal tract and stayed for over 24 hours. B . lactis BL-99 promoted the intestinal motility and effectively alleviated aluminum sulfate-induced larval zebrafish constipation ( p 〈 0.01). Irregular high glucose diet induced adult zebrafish intestinal functional and metabolic disorders. After fed with B . lactis BL-99, IL-1β gene expression was significantly down-regulated, and IL-10 and IL-12 gene levels were markedly up-regulated in this model ( p 〈 0.05). The intestinal lipase activity was elevated in the adult zebrafish intestinal functional disorder model after B . lactis BL-99 treatment ( p 〈 0.05), but tryptase content had no statistical changes ( p 〉 0.05). B . lactis BL-99 improved the histopathology of the adult zebrafish intestinal inflammation, increased the goblet cell numbers, and up-and-down metabolites were markedly recovered after treatment of B . lactis BL-99 ( p 〈 0.05). These results suggest that B . lactis BL-99 could relieve intestinal inflammation and promote intestinal functions, at least in part, through modulating intestinal and microbial metabolism to maintain intestinal health.
    Type of Medium: Online Resource
    ISSN: 1932-6203
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.1371/journal.pone.0262942
    DOI: 10.1371/journal.pone.0262942.g001
    DOI: 10.1371/journal.pone.0262942.g002
    DOI: 10.1371/journal.pone.0262942.g003
    DOI: 10.1371/journal.pone.0262942.g004
    DOI: 10.1371/journal.pone.0262942.g005
    DOI: 10.1371/journal.pone.0262942.g006
    DOI: 10.1371/journal.pone.0262942.g007
    DOI: 10.1371/journal.pone.0262942.t001
    DOI: 10.1371/journal.pone.0262942.t002
    DOI: 10.1371/journal.pone.0262942.s001
    Language: English
    Publisher: Public Library of Science (PLoS)
    Publication Date: 2022
    detail.hit.zdb_id: 2267670-3
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  • 9
    Online Resource
    Online Resource
    Lactobacillus paracasei ET-22 Suppresses Dental Caries by Regulating Microbiota of Dental Plaques and Inhibiting Biofilm Formation
    MDPI AG ; 2023
    In:  Nutrients Vol. 15, No. 15 ( 2023-07-26), p. 3316-
    Details
    In: Nutrients, MDPI AG, Vol. 15, No. 15 ( 2023-07-26), p. 3316-
    Abstract: Dental caries is a common and multifactorial biofilm disease that is associated with dietary habits and microbiota. Among the various pathogens inducing caries, S. mutans is the most extensively studied. Promoting oral health with probiotics has gained considerable attention. Lactobacillus paracasei (L. paracasei) strains were reported to modulate the gut microbiota and enhance host resistance to disease. Our previous research has found that L. paracasei ET-22 (ET-22) could inhibit S. mutans biofilms in vitro. However, the preventive effect in vivo and functional mechanism of ET-22 on dental caries were unclear. In this study, the preventive effects of ET-22 on dental caries in mice were checked. Meanwhile, the functional mechanism of ET-22 was further investigated. Results showed that the supplementation of ET-22 in drinking water significantly improved the caries scoring of mice. The microbiota of dental plaques revealed that the live and heat-killed ET-22 similarly regulated the microbial structure in plaque biofilms. Functional prediction of PICRUSt showed that the addition of live and heat-killed ET-22 may inhibit biofilm formation. By the in vitro trials, the live and heat-killed ET-22 indeed inhibited the construction of S. mutans biofilms and EPS productions of biofilms. This evidence suggests that ET-22 can restrain dental caries by regulating the microbiota of dental plaques and inhibiting biofilm formation, which may be partly mediated by the body components of ET-22.
    Type of Medium: Online Resource
    ISSN: 2072-6643
    URL: Article
    DOI: 10.3390/nu15153316
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2518386-2
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