In:
Animal Science Journal, Wiley, Vol. 89, No. 10 ( 2018-10), p. 1459-1467
Abstract:
Lactic acid bacteria were screened for potential probiotics for use as feed additives. We obtained 3,000 isolates from feces of: cattle, dogs, goats, and infants; milk; yogurt; cheese; fermented sausages; Kimchi ; and Cheonggukjang and tested their antibacterial activity toward indicator pathogens, including Bacillus cereus , Listeria monocytogenes , Escherichia coli O157:H7, and Salmonella enterica Enteritidis. We further tested their tolerance to artificial gastric juice (1% [w/v] pepsin, pH 2.5) and bile acid (0.1% [w/v] oxgall, pH 6.8). Six isolates exhibited strong antibacterial activity against indicator pathogens. The PA 40 isolate from Kimchi exhibited marked resistance to artificial gastric juice and bile acid. The antibacterial substances produced by PA 40 were stable to heat, pH , and enzymes. Strain PA 40 was identified as a Lactobacillus curvatus strain using chemical tests and 16S rDNA sequencing and produced 248.4 mmol/L lactic acid after 48 hr of fermentative growth. The L. curvatus PA 40 strain was also highly tolerant of the artificial gastrointestinal model system. Our results indicate that L. curvatus PA 40 could be used as a potential probiotic feed additive.
Type of Medium:
Online Resource
ISSN:
1344-3941
,
1740-0929
DOI:
10.1111/asj.2018.89.issue-10
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2095161-9
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