In:
Journal of Veterinary Medicine, Series B, Wiley, Vol. 45, No. 1-10 ( 1998-01-12), p. 25-29
Kurzfassung:
Acid treatment of bovine lymphocytes by a buffered solution of 0.263 m citric acid and 0.123 M Na 2 HPO 4 at pH = 3.0, originally described for human and murine lymphocytes, selectively eliminated the antigenicity of MHC (BoLA) class I determinants also from bovine lymphocytes. The viability of acid‐treated cell suspension was higher than 90%. The reactivity of acid‐treated lymphocytes with BoLA class I typing alloantisera was lost in microcytotoxicity test, while their reactions with cross‐reactive anti‐HLA class II, anti‐BoCD2 and BoCD5 monoclonal antibodies, and with antisera detecting two non‐MHC lymphocyte alloantigenic specificities (BoLY w1 and R') remained unchanged in the microcytotoxicity and/or indirect immunofluorescence tests. The results thus show that this approach of modulating cell surface expression of MHC class I determinants may be used in cattle.
Materialart:
Online-Ressource
ISSN:
0931-1793
DOI:
10.1111/zph.1998.45.issue-1-10
DOI:
10.1111/j.1439-0450.1998.tb00762.x
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
1998
ZDB Id:
2271118-1
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