In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 9 ( 2000-04-25), p. 4736-4741
Abstract:
The genome of Caenorhabditis
elegans possesses two genes, dpy-18 and phy-2, that encode α subunits of the enzyme prolyl
4-hydroxylase. We have generated deletions within each gene to eliminate prolyl 4-hydroxylase activity from the animal. The dpy-18 mutant has an aberrant body morphology,
consistent with a role of prolyl 4-hydroxylase in formation of the body cuticle. The phy-2 mutant is phenotypically wild type.
However, the dpy-18; phy-2 double mutant is not viable,
suggesting an essential role for prolyl 4-hydroxylase that is normally accomplished by either dpy-18 or phy-2 .
The effects of the double mutation were mimicked by small-molecule inhibitors of prolyl 4-hydroxylase, validating the genetic results and
suggesting that C. elegans can serve as a model system
for the discovery of new inhibitors.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.97.9.4736
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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