In:
The Open Dentistry Journal, Bentham Science Publishers Ltd., Vol. 9, No. 1 ( 2015-12-31), p. 486-491
Abstract:
Objectives : Cold plasma (CP) has been shown to be effective
even against multiresistant microorganisms. As previous investigations on the effect of CP in root canals showed promising results, the aim of the present
study was to analyze the bactericidal efficacy of CP in different depths of infected dentin. Methods : 32 standardized root canals of human
mandibular premolars were infected with Enterococcus faecalis and
incubated for one week. Specimens were randomly selected for one of four disinfection methods: control (5mL NaCl), 5mL chlorhexidine (CHX), CP alone
(CP), and a combination of 5mL CHX and cold plasma (CHX+CP). CHX was ultrasonically activated for 30s, while cold plasma was used for 60s in the root
canals. Dentin samples at depths of 300, 500 and 800 µm were obtained and diluted serially. Colony forming units (CFUs) were counted on agar plates after
24h of incubation. Results : The highest overall logarithmic
reduction factors (RF) were obtained from CHX+CP (log RF 3.56 p 〈 0.01;
Mann-Whitney U test), followed by CP (log RF 3.27 p 〈 0.01) and CHX alone (log RF
2.65 p 〈 0.01) related to the control. All disinfection methods showed
significantly lower CFU counts compared to the control group in 300 µm and 800 µm (both p 〈 0.01, Kruskal-Wallis test). Discussion : The adjuvant use of CP might be beneficial in highly
infected root canals to improved disinfection. However, the disinfection effect against Enterococcus faecalis of CP is comparable to ultrasonically
activated CHX.
Type of Medium:
Online Resource
ISSN:
1874-2106
DOI:
10.2174/1874210601509010486
Language:
English
Publisher:
Bentham Science Publishers Ltd.
Publication Date:
2015
detail.hit.zdb_id:
2396267-7
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