In:
European Journal of Immunology, Wiley, Vol. 8, No. 4 ( 1978-04), p. 228-236
Abstract:
The nature of the target antigen, expressed on murine sarcoma virus (MSV) and related murine tumors which reacts with T killer lymphocytes, remains ill‐defined. The experiments reported here show that: (a) the previously described H‐2 restriction phenomenon is found under all experimental conditions including 3–4 and 16–20‐h chromium release tests. With 16–20 h tests and highly efficient T lymphocytes however, quantitative methods are necessary to demonstrate the H‐2 restriction. These results support the hypothesis that H‐2 molecules may be determinant in the structure and/or in the function of the cytolytic T lymphocyte (CTL) reactive antigen. (b) Under syngeneic conditions ( i.e. using H‐2‐identical immune lymphocytes, stimulators and target cells), the pattern of specificities recognized by anti‐MSV or anti‐Friend T killer cells on 20 different lymphomas suggests that the main reactive antigen is an “FMR‐like” substance. Identical conclusions were drawn from competition experiments. (c) Blockings were obtained by pre‐incubation of the target cells with a goat anti‐gp70, suggesting a possible role of the viral gp70 in the antigen recognized. However, this could be due to nonspecific reactions as two other anti‐gp70 sera as well as with antisera directed against the viral components gp45, pr60, p30, p15, p12 and p10 did not block. The CTL/tumor cell interaction was not inhibited by virion‐associated antigens added to the medium. Lysostrip and co‐capping experiments have failed to reveal an association between H‐2 and gp70. The nature of the viral protein bearing the “FMR‐like” substance therefore remains to be established.
Type of Medium:
Online Resource
ISSN:
0014-2980
,
1521-4141
DOI:
10.1002/eji.1830080403
Language:
English
Publisher:
Wiley
Publication Date:
1978
detail.hit.zdb_id:
120108-6
detail.hit.zdb_id:
1491907-2
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