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  • 1
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    Online Resource
    Database Resources of the National Genomics Data Center, China National Center for Bioinformation in 2021
    Oxford University Press (OUP) ; 2021
    In:  Nucleic Acids Research Vol. 49, No. D1 ( 2021-01-08), p. D18-D28
    Details
    In: Nucleic Acids Research, Oxford University Press (OUP), Vol. 49, No. D1 ( 2021-01-08), p. D18-D28
    Abstract: The National Genomics Data Center (NGDC), part of the China National Center for Bioinformation (CNCB), provides a suite of database resources to support worldwide research activities in both academia and industry. With the explosive growth of multi-omics data, CNCB-NGDC is continually expanding, updating and enriching its core database resources through big data deposition, integration and translation. In the past year, considerable efforts have been devoted to 2019nCoVR, a newly established resource providing a global landscape of SARS-CoV-2 genomic sequences, variants, and haplotypes, as well as Aging Atlas, BrainBase, GTDB (Glycosyltransferases Database), LncExpDB, and TransCirc (Translation potential for circular RNAs). Meanwhile, a series of resources have been updated and improved, including BioProject, BioSample, GWH (Genome Warehouse), GVM (Genome Variation Map), GEN (Gene Expression Nebulas) as well as several biodiversity and plant resources. Particularly, BIG Search, a scalable, one-stop, cross-database search engine, has been significantly updated by providing easy access to a large number of internal and external biological resources from CNCB-NGDC, our partners, EBI and NCBI. All of these resources along with their services are publicly accessible at https://bigd.big.ac.cn.
    Type of Medium: Online Resource
    ISSN: 0305-1048 , 1362-4962
    URL: Article
    DOI: 10.1093/nar/gkaa1022
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2021
    detail.hit.zdb_id: 1472175-2
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  • 2
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    Automatic Identification of Individual Primates with Deep Learning Techniques
    Elsevier BV ; 2020
    In:  iScience Vol. 23, No. 8 ( 2020-08), p. 101412-
    Details
    In: iScience, Elsevier BV, Vol. 23, No. 8 ( 2020-08), p. 101412-
    Type of Medium: Online Resource
    ISSN: 2589-0042
    URL: Article
    DOI: 10.1016/j.isci.2020.101412
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2020
    detail.hit.zdb_id: 2927064-9
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  • 3
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    Anomalous inflation of a nematic balloon
    Elsevier BV ; 2020
    In:  Journal of the Mechanics and Physics of Solids Vol. 142 ( 2020-09), p. 104013-
    Details
    In: Journal of the Mechanics and Physics of Solids, Elsevier BV, Vol. 142 ( 2020-09), p. 104013-
    Type of Medium: Online Resource
    ISSN: 0022-5096
    URL: Article
    DOI: 10.1016/j.jmps.2020.104013
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2020
    detail.hit.zdb_id: 2012341-3
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  • 4
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    Uniaxial tension of a nematic elastomer with inclined mesogens
    Elsevier BV ; 2020
    In:  Extreme Mechanics Letters Vol. 40 ( 2020-10), p. 100936-
    Details
    In: Extreme Mechanics Letters, Elsevier BV, Vol. 40 ( 2020-10), p. 100936-
    Type of Medium: Online Resource
    ISSN: 2352-4316
    URL: Article
    DOI: 10.1016/j.eml.2020.100936
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2020
    detail.hit.zdb_id: 2810750-0
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  • 5
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    Numerical simulation of impact crater formation and distribution of high-pressure polymorphs
    Elsevier BV ; 2023
    In:  Acta Astronautica Vol. 203 ( 2023-02), p. 169-186
    Details
    In: Acta Astronautica, Elsevier BV, Vol. 203 ( 2023-02), p. 169-186
    Type of Medium: Online Resource
    ISSN: 0094-5765
    URL: Article
    DOI: 10.1016/j.actaastro.2022.11.048
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2014614-0
    SSG: 16,12
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  • 6
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    Numerical Study on Asteroid Deflection by Penetrating Explosion Based on Single-Material ALE Method and FE-SPH Adaptive Method
    MDPI AG ; 2023
    In:  Aerospace Vol. 10, No. 5 ( 2023-05-18), p. 479-
    Details
    In: Aerospace, MDPI AG, Vol. 10, No. 5 ( 2023-05-18), p. 479-
    Abstract: An asteroid impact can potentially destroy life on this planet. Therefore, asteroids should be prevented from impacting the Earth to impede severe disasters. Nuclear explosions are currently the only option to prevent an incoming asteroid impact when the asteroid is large or the warning time is short. However, asteroids exist in an absolute vacuum, where the explosion energy propagation mechanism differs from that in an air environment. It is difficult to describe this process using standard numerical simulation methods. In this study, we used the single-material arbitrary Lagrangian–Eulerian (ALE) method and the finite element-smoothed particle hydrodynamics (FE-SPH) adaptive method to simulate the process of deflecting hazardous asteroids using penetrating explosions. The single-material ALE method can demonstrate the expansion process of explosion products and energy coupling in absolute vacuum. The FE-SPH adaptive method can transform failed elements into SPH particles during the simulation, avoiding system mass loss, energy loss, and element distortion. We analyzed the shock initiation and explosion damage process and obtained an effective simulation of the damage evolution, stress propagation, and fragment distribution of the asteroid. In addition, we decoupled the penetrating explosion into two processes: kinetic impact and static explosion at the impact crater. The corresponding asteroid damage modes, velocity changes, and fragmentation degrees were simulated and compared. Finally, the high efficiency of the nuclear explosion was confirmed by comparing the contribution rates of the kinetic impact and nuclear explosion in the penetrating explosion scheme.
    Type of Medium: Online Resource
    ISSN: 2226-4310
    URL: Article
    DOI: 10.3390/aerospace10050479
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2756091-0
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  • 7
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    Arabidopsis membrane protein AMAR1 interaction with type III effector XopAM triggers a hypersensitive response
    Oxford University Press (OUP) ; 2023
    In:  Plant Physiology ( 2023-08-30)
    Details
    In: Plant Physiology, Oxford University Press (OUP), ( 2023-08-30)
    Abstract: The efficient infection of plants by the bacteria Xanthomonas campestris pv. campestris (Xcc) depends on its type III effectors (T3Es). Although the functions of AvrE family T3Es have been reported in some bacteria, the member XopAM in Xcc has not been studied. As XopAM has low sequence similarity to reported AvrE-T3Es and different reports have shown that these T3Es have different targets in hosts, we investigated the functions of XopAM in the Xcc–plant interaction. Deletion of xopAM from Xcc reduced its virulence in cruciferous crops but increased virulence in Arabidopsis (Arabidopsis thaliana) Col-0, indicating that XopAM may perform opposite functions depending on the host species. We further found that XopAM is a lipase that may target the cytomembrane and that this activity might be enhanced by its membrane-targeted protein XOPAM-ACTIVATED RESISTANCE 1 (AMAR1) in Arabidopsis Col-0. The binding of XopAM to AMAR1 induced an intense hypersensitive response that restricted Xcc proliferation. Our results showed that the roles of XopAM in Xcc infection are not the same as those of other AvrE-T3Es, indicating that the functions of this type of T3E have differentiated during long-term bacterium‒host interactions.
    Type of Medium: Online Resource
    ISSN: 0032-0889 , 1532-2548
    URL: Article
    DOI: 10.1093/plphys/kiad478
    RVK:
    WA 15000
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2023
    detail.hit.zdb_id: 2004346-6
    detail.hit.zdb_id: 208914-2
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  • 8
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    Laparoscopic specimen extraction in vitro: preliminary experience
    Springer Science and Business Media LLC ; 2021
    In:  BMC Surgery Vol. 21, No. 1 ( 2021-12)
    Details
    In: BMC Surgery, Springer Science and Business Media LLC, Vol. 21, No. 1 ( 2021-12)
    Abstract: The last procedure performed by the surgeon in laparoscopic surgery is to extract the specimen through the smallest incision possible. This experiment aimed to explore the maximum diameter of specimens that can be extracted through auxiliary incisions of different lengths and shapes by in vitro physical experiments. Materials and methods We used the abdominal wall with the muscle layer, fixed on a square wooden frame, to simulate the human abdominal wall. Then, specimen extraction ports were made with circular, inverted Y-shaped and straight-line incisions of different sizes and lengths, and specimens of different sizes were made from tissues of different species. These specimens were extracted from different incisions with a force gauge. The tension value (N) was measured, and records were made of the length or diameter of the smallest auxiliary incision through which a given specimen could pass, as well as the largest specimen diameter that could pass through an incision of a given size. This experiment provides us with preliminary experience-based knowledge of how to choose the appropriate auxiliary incision for surgical specimen extraction according to the diameter of the specimen. Results The maximum diameters of specimens that could be extracted with circular ostomy diameters of 2.4, 2.7 and 3.3 cm were 4.0, 4.5 and 6.0 cm, respectively. Specimens with diameters of 6.0, 8.0 and 10.0 cm could be extracted through inverted Y-shaped incisions with a length around the umbilicus of 1 cm and an extension length of 1.0, 3.0, and 4.0 cm, respectively. Moreover, these same specimens could be extracted through inverted Y-shaped incisions with a length around the umbilicus of 2 cm and extension lengths of 0.0, 1.0 and 2.0 cm. Tough tissue specimens (made from chicken gizzards) with diameters of 1.0, 2.0, 4.0 and 6.0 cm, respectively, could be removed through straight-line incisions measuring 1.0, 2.0, 3.0 and 4.0 cm in length. Conclusion Along with preoperative imaging, surgical planning and trocar position, the shape and length of auxiliary incisions can be used to improve the extraction of specimens via laparoscopic surgery.
    Type of Medium: Online Resource
    ISSN: 1471-2482
    URL: Article
    DOI: 10.1186/s12893-021-01300-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2050442-1
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  • 9
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    Transcriptome-based discovery of pathways and genes related to resistance against Fusariumhead blight in wheat landrace Wangshuibai
    Springer Science and Business Media LLC ; 2013
    In:  BMC Genomics Vol. 14, No. 1 ( 2013-12)
    Details
    In: BMC Genomics, Springer Science and Business Media LLC, Vol. 14, No. 1 ( 2013-12)
    Abstract: Fusarium head blight (FHB), caused mainly by Fusarium graminearum (Fg) Schwabe (teleomorph: Gibberellazeae Schwble), brings serious damage to wheat production. Chinese wheat landrace Wangshuibai is one of the most important resistance sources in the world. The knowledge of mechanism underlying its resistance to FHB is still limited. Results To get an overview of transcriptome characteristics of Wangshuibai during infection by Fg , a high-throughput RNA sequencing based on next generation sequencing (NGS) technology (Illumina) were performed. Totally, 165,499 unigenes were generated and assigned to known protein databases including NCBI non-redundant protein database (nr) (82,721, 50.0%), Gene Ontology (GO) (38,184, 23.1%), Swiss-Prot (50,702, 30.6%), Clusters of orthologous groups (COG) (51,566, 31.2%) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) (30,657, 18.5%), as determined by Blastx search. With another NGS based platform, a digital gene expression (DGE) system, gene expression in Wangshuibai and its FHB susceptible mutant NAUH117 was profiled and compared at two infection stages by inoculation of Fg at 24 and 48 hour, with the aim of identifying genes involved in FHB resistance. Conclusion Pathogen-related proteins such as PR5 , PR14 and ABC transporter and JA signaling pathway were crucial for FHB resistance, especially that mediated by Fhb1 . ET pathway and ROS/NO pathway were not activated in Wangshuibai and may be not pivotal in defense to FHB. Consistent with the fact that in NAUH117 there presented a chromosome fragment deletion, which led to its increased FHB susceptibility, in Wangshuibai, twenty out of eighty-nine genes showed changed expression patterns upon the infection of Fg . The up-regulation of eight of them was confirmed by qRT-PCR, revealing they may be candidate genes for Fhb1 and need further functional analysis to confirm their roles in FHB resistance.
    Type of Medium: Online Resource
    ISSN: 1471-2164
    URL: Article
    DOI: 10.1186/1471-2164-14-197
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2013
    detail.hit.zdb_id: 2041499-7
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  • 10
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    Development of SNP-Based Markers to Identify Colletotrichum gossypii in Upland Cotton
    Scientific Societies ; 2018
    In:  Plant Disease Vol. 102, No. 7 ( 2018-07), p. 1426-1433
    Details
    In: Plant Disease, Scientific Societies, Vol. 102, No. 7 ( 2018-07), p. 1426-1433
    Abstract: Colletotrichum gossypii is a causal agent of anthracnose in upland cotton (Gossypii hirsutum). C. gossypii belongs to the C. gloeosporioides species complex. However, the high level of genetic similarity among the species within the C. gloeosporioides species complex makes the development of a diagnostic assay for C. gossypii challenging. Furthermore, the spore size and cultural characteristics of C. gossypii from different geographical areas can vary. In this study, we examined the morphological variance, growth pattern, and pathogenicity of C. gossypii and developed a molecular diagnostic assay to detect C. gossypii in cotton plants from different regions of China. To overcome any ambiguity in morphological and pathogenic characteristics, a set of primers targeting the β-tubulin (TUB) gene of C. gossypii was designed. Single-nucleotide polymorphisms (SNP) were used to identify C. gossypii at the species level using DNA sequence alignments of the TUB gene. The SPSCG/F and SPSCG/R primer pair only amplified C. gossypii, and was able to amplify C. gossypii in mixtures of other Colletotrichum spp., even when DNA concentrations were up to 10 times lower than that of the other species. This is the first report of the development of SNP-based markers for the specific identification of C. gossypii.
    Type of Medium: Online Resource
    ISSN: 0191-2917 , 1943-7692
    URL: Article
    DOI: 10.1094/PDIS-10-17-1672-RE
    Language: English
    Publisher: Scientific Societies
    Publication Date: 2018
    detail.hit.zdb_id: 2042679-3
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