In:
Journal of Chemistry, Hindawi Limited, Vol. 2018 ( 2018), p. 1-9
Abstract:
Gamma-tocotrienol, a major component of tocotrienol-rich fraction of palm oil, has been suggested to exhibit bone protective effects in vivo . However, the effects of γ -tocotrienol on osteoblast cells are still unclear. In this study, the effects of γ -tocotrienol on the proliferation, differentiation, and mineralization in osteoblastic MC3T3-E1 cells were investigated. Our results showed that γ -tocotrienol (2–8 μ mol/L) significantly improved the cell proliferation ( p 〈 0 . 05 ), but it did not affect cell cycle progression. γ -Tocotrienol significantly increased alkaline phosphatase (ALP) activity ( p 〈 0 . 05 ), secretion levels of osteocalcin (OC) and osteonectin (ON), and mRNA levels of collagen type I (Col I) of MC3T3-E1 cells. Meanwhile, we found that γ -tocotrienol is promoted in differentiation MC3T3-E1 cells by upregulation of the expression of Runx2 protein. Moreover, the number of bone nodules increased over 2.5-fold in cells treated with γ -tocotrienol (2–8 μ mol/L) for 24 d compared to control group. These results indicated that γ -tocotrienol at low dose levels, especially 4 μ mol/L, could markedly enhance the osteoblastic function by increasing the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells. Moreover, our data also indicated that Runx2 protein may be involved in these effects. Further studies are needed to determine the potential of γ -tocotrienol as an antiosteoporotic agent.
Type of Medium:
Online Resource
ISSN:
2090-9063
,
2090-9071
DOI:
10.1155/2018/3805932
Language:
English
Publisher:
Hindawi Limited
Publication Date:
2018
detail.hit.zdb_id:
2393625-3
detail.hit.zdb_id:
2703077-5
Permalink