In:
Journal of Basic Microbiology, Wiley, Vol. 63, No. 9 ( 2023-09), p. 1030-1048
Abstract:
The oral antimicrobial and cytotoxic properties of green synthesized novel titanium dioxide nanoparticles (TiO 2 NPs) using Iranian propolis extracts were investigated on oral bacteria and fibroblast cells. In this study, propolis was sampled, and alcoholic extracts were prepared. The TiO 2 NPs were biosynthesized using propolis extracts. The synthesized TiO 2 NPs were characterized by scanning electron microscope (SEM), X‐ray diffraction analysis, energy‐dispersive X‐ray (EDX), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering, ultraviolet–visible (UV–Vis), transmission electron microscope, Brunauer–Emmett–Teller, and zeta potential. MTT (3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyl tetrazolium bromide), minimal inhibitory concentration, minimum bactericidal concentration, minimum fungicidal concentration, biofilm formation, and degradation tests were studied to clarify the oral antimicrobial properties of green synthesized TiO 2 NPs. According to the FTIR analysis, the propolis extract contained flavonoids and phenolic compounds in addition to TiO 2 NPs. Additionally, UV‐Vis revealed that intense bands had formed NPs. EDX spectra and SEM images revealed that the stabilizing agent was in perfect quasi‐spherical shapes around 21 nm. An EDX spectrum was used to verify the presence of titanium and oxygen. There were no significant cytotoxicity effects. The antibacterial results showed that Pro1TiO 2 (Khalkhal sample) had better effects than Pro2TiO 2 (Gilan sample) and TiO 2 NPs. The present study presents a new process for synthesizing TiO 2 NPs from propolis extracts with less toxic effects and user‐friendly, eco‐friendly, and economical materials. Pro1TiO 2 NPs may be considered the best candidate for clinical application.
Type of Medium:
Online Resource
ISSN:
0233-111X
,
1521-4028
DOI:
10.1002/jobm.202300221
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
1480967-9
detail.hit.zdb_id:
632513-0
detail.hit.zdb_id:
203025-1
SSG:
12
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