In:
Molecular Nutrition & Food Research, Wiley, Vol. 58, No. 10 ( 2014-10), p. 2080-2093
Kurzfassung:
Immunobiotics are known to modulate intestinal immune responses by regulating T oll‐like receptor ( TLR ) signaling pathways, which are responsible for the induction of cytokines and chemokines in response to microbial‐associated molecular patterns. However, little is known about the immunomodulatory activity of compounds or molecules from immunobiotics. Methods and results We evaluated whether L actobacillus delbrueckii subsp. delbrueckii TUA 4408L (Ld) or its extracellular polysaccharide ( EPS ): acidic EPS ( APS ) and neutral EPS ( NPS ), modulated the response of porcine intestinal epitheliocyte ( PIE ) cells against Enterotoxigenic E scherichia coli ( ETEC ) 987P. The roles of TLR 2, TLR 4, and TLR negative regulators in the immunoregulatory effects were also studied. ETEC ‐induced inflammatory cytokines were downregulated when PIE cells were prestimulated with both Ld or EPS s. Ld, APS , and NPS inhibited ETEC mediated mitogen‐activated protein kinase ( MAPK ) and nuclear factor‐κB ( NF ‐κB) activation by upregulating TLR negative regulators. The capability of Ld to suppress inflammatory cytokines was diminished when PIE cells were blocked with anti‐ TLR 2 antibody, while APS failed to suppress inflammatory cytokines when cells were treated with anti‐ TLR 4 antibody. Induction of Ca 2+ fluxes in TLR knockdown cells confirmed that TLR 2 plays a principal role in the immunomodulatory action of Ld, while the activity of APS is mediated by TLR 4. In addition, NPS activity depends on both TLR 4 and TLR 2. Conclusion Ld and its EPS have the potential to be used for the development of anti‐inflammatory functional foods to prevent intestinal diseases in both humans and animals.
Materialart:
Online-Ressource
ISSN:
1613-4125
,
1613-4133
DOI:
10.1002/mnfr.201400218
Sprache:
Englisch
Verlag:
Wiley
Publikationsdatum:
2014
ZDB Id:
2160372-8
SSG:
12
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