In:
The Journal of Immunology, The American Association of Immunologists, Vol. 182, No. 1_Supplement ( 2009-04-01), p. 133.40-133.40
Abstract:
Infection of immature dendritic cells (DCs) by virus stimulates their maturation into antigen presenting cells (APC). Infected DCs secrete TNFα, IFNα, IFNβ, MIP1α, RANTES, IL-6 and IL-8 which expose uninfected DCs to this complex cytokines/chemokines milieu. Recently, we showed that this paracrine signaling from infected DCs induced an antiviral-primed DC state referred to as antiviral activated DCs (AVDCs, J Immunology 2008 PMID: 18981106). AVDCs are relatively resistant to virus infection in comparison with naïve DCs and achieve accelerated and augmented levels of co-stimulatory molecule expression with virus infection. Here we investigate the factors reponsible for AVDC formation. No single factor induces a similar surface marker pattern in DCs as observed in AVDCs. The effects of all possible double combinations on naive DCs have been evaluated by RNA expression pattern, surface marker expression, as well as imaging flow analysis of rate of phagocytosis and DC-T cell interaction. The results illuminate how DCs integrate multiple paracrine signals to modulate their antiviral state.
Type of Medium:
Online Resource
ISSN:
0022-1767
,
1550-6606
DOI:
10.4049/jimmunol.182.Supp.133.40
Language:
English
Publisher:
The American Association of Immunologists
Publication Date:
2009
detail.hit.zdb_id:
1475085-5
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