In:
International Journal of Dermatology, Wiley, Vol. 54, No. 1 ( 2015-01)
Abstract:
Langerhans cell histiocytosis ( LCH ) is a rare and potentially fatal disorder of unknown etiology arising from the accumulation of epidermal L angerhans‐like cells in bone, skin, or other tissues. Tissue damage and morbidity results from lesional cytokine release, and we sought to investigate the LCH microenvironment using a combination of histological stains and immunohistochemistry. Methods CD 1a immunoreactivity was used to identify lesional cells in archival paraffin‐embedded samples of cutaneous LCH . A combined Orcein and G iemsa stain identified immune cells in general (particularly granulocytes and mast cells) and extracellular matrix (particularly elastic fibers), while CD 3 and CD 68 staining identified T cells and macrophages, respectively. Collagen synthesis was investigated with H erovici staining, which discriminates newly synthesized from mature collagen, while cross‐polar microscopy of picrosirius‐stained sections identified changes in matrix organization. Results Immune cells were consistently identified at the periphery of cutaneous LCH lesions. We quantified an increased number of thickened and disorganized elastic fibers surrounding lesions and an absence of elastic fibers within lesions. Furthermore, lesions exhibited a decrease in mature collagen fibers and a loss of supporting collagen matrix within lesions and compromised collagen integrity in adjacent tissue. Conclusions Cutaneous LCH lesions are associated with the peripheral recruitment of a variety of immune cells and are consistently characterized by localized elastosis, collagen damage, and remodeling.
Type of Medium:
Online Resource
ISSN:
0011-9059
,
1365-4632
DOI:
10.1111/ijd.2014.54.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2020365-2
Permalink