In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-07-02)
Abstract:
The plant hormone auxin is essential for plant growth and development. YUCCA proteins catalyse the rate-limiting step for endogenous auxin biosynthesis. In this study, we isolated 20 MdYUCCA genes from apple genome. MdYUCCA6a , MdYUCCA8a , and MdYUCCA10a were expressed in most organs and could support whole plant basal auxin synthesis. MdYUCCA4a , MdYUCCA10b , and MdYUCCA11a expression indicated roles for these genes in auxin biosynthesis in vegetative organs. MdYUCCA2b , MdYUCCA11b , and MdYUCCA11d were mainly expressed in flower organs. High temperature induced the expression of MdYUCCA4a , MdYUCCA6a , MdYUCCA8a , and MdYUCCA10a , and down-regulated the expression of MdYUCCA2b and MdYUCCA6b . Dual-luciferase assay indicated that MdPIF4 could trans-activate the MdYUCCA8a promoter. Overexpression of MdYUCCA8a increased IAA content, increased stem height, enhanced apical dominance, and led to silique malformation. These results provide a foundation for further investigation of the biological functions of apple MdYUCCAs .
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-020-66483-y
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2020
detail.hit.zdb_id:
2615211-3
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