In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 62, No. Pt_11 ( 2012-11-01), p. 2685-2690
Abstract:
Two novel obligately anaerobic, Gram-stain-positive, saccharolytic and non-proteolytic spore-forming bacilli (strains CD3 : 22 T and N1 T ) are described. Strain CD3 : 22 T was isolated from a biopsy of the small intestine of a child with coeliac disease, and strain N1 T from the saliva of a healthy young man. The cells of both strains were observed to be filamentous, approximately 5 to 〉 20 µm long, some of them curving and with swellings. The novel organisms produced H 2 S, NH 3 , butyric acid and acetic acid as major metabolic end products. Phylogenetic analyses, based on comparative 16S rRNA gene sequencing, revealed close relationships (98 % sequence similarity) between the two isolates, as well as the type strain of Eubacterium saburreum and four other Lachnospiraceae bacterium-/ E. saburreum -like organisms. This group of bacteria were clearly different from any of the 19 known genera in the family Lachnospiraceae . While Eubacterium species are reported to be non-spore-forming, reanalysis of E. saburreum CCUG 28089 T confirmed that the bacterium is indeed able to form spores. Based on 16S rRNA gene sequencing, phenotypic and biochemical properties, strains CD3 : 22 T and N1 T represent novel species of a new and distinct genus, named Lachnoanaerobaculum gen. nov., in the family Lachnospiraceae [within the order Clostridiales , class Clostridia , phylum Firmicutes ]. Strain CD3 : 22 T ( = CCUG 58757 T = DSM 23576 T ) is the type strain of the type species, Lachnoanaerobaculum umeaense gen. nov., sp. nov., of the proposed new genus. Strain N1 T ( = CCUG 60305 T = DSM 24553 T ) is the type strain of Lachnoanaerobaculum orale sp. nov. Moreover, Eubacterium saburreum is reclassified as Lachnoanaerobaculum saburreum comb. nov. (type strain CCUG 28089 T = ATCC 33271 T = CIP 105341 T = DSM 3986 T = JCM 11021 T = VPI 11763 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.033613-0
Language:
English
Publisher:
Microbiology Society
Publication Date:
2012
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
Permalink