In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 72, No. 8_Supplement ( 2012-04-15), p. LB-276-LB-276
Abstract:
siRNA is a powerful tool in loss-of-function studies which generates valuable information on target validation. siRNA is highly selective and suppress gene expression in a sequence-specific manner. These features have made it a key tool in signal transduction - a cornerstone in cancer biology. 2-D DIGE (two dimensional differential gel electrophoresis) is a fluorescence based technology with the capacity to separate thousands of proteins in one single run, with high reproducibility. This makes it a valuable tool for differential expression analyses. In this study two powerful methods, siRNA and 2-D DIGE, were combined to investigate a well-known signaling pathway, pivotal in cancer biology. Silencing of the target protein made it possible not only to identify a large number of proteins that were differentially regulated but also to resolve the time dependency of protein regulation. Thus the combined use of siRNA and 2-D DIGE made it possible to find potentially new targets in the signaling pathway. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-276. doi:1538-7445.AM2012-LB-276
Type of Medium:
Online Resource
ISSN:
0008-5472
,
1538-7445
DOI:
10.1158/1538-7445.AM2012-LB-276
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2012
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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