In:
PLOS Biology, Public Library of Science (PLoS), Vol. 19, No. 5 ( 2021-5-25), p. e3001263-
Abstract:
We here conducted an image-based chemical screen to evaluate how medically approved drugs, as well as drugs that are currently under development, influence overall translation levels. None of the compounds up-regulated translation, which could be due to the screen being performed in cancer cells grown in full media where translation is already present at very high levels. Regarding translation down-regulators, and consistent with current knowledge, inhibitors of the mechanistic target of rapamycin (mTOR) signaling pathway were the most represented class. In addition, we identified that inhibitors of sphingosine kinases (SPHKs) also reduce mRNA translation levels independently of mTOR. Mechanistically, this is explained by an effect of the compounds on the membranes of the endoplasmic reticulum (ER), which activates the integrated stress response (ISR) and contributes to the toxicity of SPHK inhibitors. Surprisingly, the toxicity and activation of the ISR triggered by 2 independent SPHK inhibitors, SKI-II and ABC294640, the latter in clinical trials, are also observed in cells lacking SPHK1 and SPHK2. In summary, our study provides a useful resource on the effects of medically used drugs on translation, identified compounds capable of reducing translation independently of mTOR and has revealed that the cytotoxic properties of SPHK inhibitors being developed as anticancer agents are independent of SPHKs.
Type of Medium:
Online Resource
ISSN:
1545-7885
DOI:
10.1371/journal.pbio.3001263
DOI:
10.1371/journal.pbio.3001263.g001
DOI:
10.1371/journal.pbio.3001263.g002
DOI:
10.1371/journal.pbio.3001263.g003
DOI:
10.1371/journal.pbio.3001263.g004
DOI:
10.1371/journal.pbio.3001263.s001
DOI:
10.1371/journal.pbio.3001263.s002
DOI:
10.1371/journal.pbio.3001263.s003
DOI:
10.1371/journal.pbio.3001263.s004
DOI:
10.1371/journal.pbio.3001263.s005
DOI:
10.1371/journal.pbio.3001263.s006
DOI:
10.1371/journal.pbio.3001263.s007
DOI:
10.1371/journal.pbio.3001263.s008
DOI:
10.1371/journal.pbio.3001263.s009
DOI:
10.1371/journal.pbio.3001263.s010
DOI:
10.1371/journal.pbio.3001263.s011
DOI:
10.1371/journal.pbio.3001263.s012
DOI:
10.1371/journal.pbio.3001263.s013
DOI:
10.1371/journal.pbio.3001263.s014
DOI:
10.1371/journal.pbio.3001263.s015
DOI:
10.1371/journal.pbio.3001263.s016
DOI:
10.1371/journal.pbio.3001263.s017
DOI:
10.1371/journal.pbio.3001263.s018
DOI:
10.1371/journal.pbio.3001263.r001
DOI:
10.1371/journal.pbio.3001263.r002
DOI:
10.1371/journal.pbio.3001263.r003
DOI:
10.1371/journal.pbio.3001263.r004
DOI:
10.1371/journal.pbio.3001263.r005
DOI:
10.1371/journal.pbio.3001263.r006
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2126773-X
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