In:
Journal of Neurochemistry, Wiley, Vol. 82, No. 6 ( 2002-09), p. 1540-1548
Abstract:
Mutations in the human presenilin genes ( PS1 or PS2 ) have been linked to autosomal dominant, early onset Alzheimer's disease (AD). Presenilins, probably as an essential part of gamma‐secretase, modulate gamma‐cleavage of the amyloid protein precursor (APP) to the amyloid β‐peptide (Aβ). Mutations in sel‐12 , a Caenorhabditis elegans presenilin homologue, cause a defect in egg laying that can be suppressed by loss of function mutations in a second gene, SEL‐10 . SEL‐10 protein is a homologue of yeast Cdc4, a member of the SCF ( S kp1‐ C dc53/CUL1‐ F ‐box protein) E2‐E3 ubiquitin ligase family. In this study, we show that human SEL‐10 interacts with PS1 and enhances PS1 ubiquitination, thus altering cellular levels of unprocessed PS1 and its N‐ and C‐terminal fragments. Co‐transfection of sel‐10 and APP cDNAs in HEK293 cells leads to an alteration in the metabolism of APP and to an increase in the production of amyloid β‐peptide, the principal component of amyloid plaque in Alzheimer's disease.
Type of Medium:
Online Resource
ISSN:
0022-3042
,
1471-4159
DOI:
10.1046/j.1471-4159.2002.01105.x
Language:
English
Publisher:
Wiley
Publication Date:
2002
detail.hit.zdb_id:
2020528-4
SSG:
12
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