In:
Journal of Oral Pathology & Medicine, Wiley, Vol. 47, No. 1 ( 2018-01), p. 40-47
Abstract:
Genetic factors play a large role in cancer, and thus, there is a great desire to understand the effects of different genes in cancer and to also develop gene therapy for better treatments. Therefore, the development of alternative diagnosis and therapy modalities is of utmost importance. The aim of our study was to illuminate the role of ESM 1 (endothelial cell‐specific molecule‐1, also known as Endocan) in proliferation and migration of head and neck cancer, thus helping to pave the way for new treatment modalities and predictive biomarkers. Methods ESM 1 expression was shown with immunofluorescence assay using confocal laser scanning microscope in primary and metastatic head and neck cancer cells. ESM 1 expression was knocked down by RNA interference in head and neck cancer cells. Knockdown efficiency was evaluated by quantitative real‐time RT ‐ PCR and Western blot. Cell proliferation and migration assays were performed by xCELL igence real‐time cell analysis system. Results Immunofluorescence assay showed nuclear localization and high expression of ESM 1 in primary and metastatic head and neck cancer cells. ESM 1 mRNA and protein levels were significantly decreased in ESM 1‐knockdown cells compared to control. ESM 1‐knockdown cells showed reduced proliferation and migration activity when compared to control cells. Conclusion These findings suggest that ESM 1 has roles on proliferation and migration of head and neck cancer cells.
Type of Medium:
Online Resource
ISSN:
0904-2512
,
1600-0714
DOI:
10.1111/jop.2018.47.issue-1
Language:
English
Publisher:
Wiley
Publication Date:
2018
detail.hit.zdb_id:
2026385-5
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