In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 65, No. Pt_5 ( 2015-05-01), p. 1653-1658
Abstract:
Yellow or orange-to-brown pigmented, ovoid or rod-shaped, Gram-negative staining, aerobic strains PE 4-5 T and N5-10 m-1 were isolated from brackish water in Lake Peng Co and fresh to brackish water in Lake Namtso on the Tibetan Plateau, China. Bacteriochlorophyll a was produced by the isolates. The predominant cellular fatty acids were C 16 : 1 , C 17 : 1 and C 18 : 1 unsaturated fatty acids, C 17 : 1 ω6 c (55.3 %), C 17 : 1 ω8 c (13.0 %) and C 18 : 1 ω7 c (10.4 %) for PE 4-5 T and C 18 : 1 ω7 c (54.7 %) and C 16 : 1 ω7 c (18.0 %) for N5-10 m-1. The polar lipid profiles of strains PE 4-5 T and N5-10 m-1 were composed of diphosphatidylglycerol, phosphatidylcholine (not detected in N5-10 m-1), phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, sphingoglycolipid and an unknown phospholipid. The predominant respiratory quinone was ubiquinone Q10 and the DNA G+C content was 66.0 mol% for both strains. The16S rRNA gene sequence of strain PE 4-5 T shared 99.0 % similarity with that of N5-10 m-1, and 97.56 % similarity with those of Blastomonas natatoria LMG 17322 T and Blastomonas ursincola DSM 9006 T , respectively. The DNA–DNA hybridization relatedness between strains PE 4-5 T and N5-10 m-1 was 79.0±1.0 %, but below 70 % with the type strains in the genus Blastomonas . Based on the variability of phylogenetic and phenotypic characteristics, the isolates should be classified as representatives of a novel species of the genus Blastomonas ; the name Blastomonas aquatica sp. nov. is proposed. The type strain is PE 4-5 T ( = JCM 30179 T = CGMCC 1.12851 T ).
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijs.0.000153
Language:
English
Publisher:
Microbiology Society
Publication Date:
2015
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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