In:
E3S Web of Conferences, EDP Sciences, Vol. 271 ( 2021), p. 03067-
Abstract:
Currently, the laboratory diagnostic tests available for HIV-1 viral infection are mainly based on serological testing which relies on enzyme-linked immunosorbent assay (ELISA) for blood HIV antigen detection and reverse transcription polymerase chain reaction (RT-PCR) for HIV specific RNA sequence identification. However, these methods are expensive and time-consuming, and suffer from false positive and/or false negative results. Thus, there is an urgent need for developing a cost effective, rapid and accurate diagnostic method for HIV-1 infection. In order to reduce the barriers for effective diagnosis, a near-infrared spectroscopy (NIR) method was used to detect the HIV-1 virus in human serum, specifically, three absorption peaks with dose-dependent at 1582nm, 1810nm and 2363nm were found by multiple FBiPLSR test analysis for HIV-nano and HIV-EGFP, but not for MLV. Therefore, we recommend the use of 1582nm, 1810nm and 2363nm as the characteristic spectrum peak, for early screening and rapid diagnosis of serum HIV.
Type of Medium:
Online Resource
ISSN:
2267-1242
DOI:
10.1051/e3sconf/202127103067
Language:
English
Publisher:
EDP Sciences
Publication Date:
2021
detail.hit.zdb_id:
2755680-3
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