In:
Journal of Oral Pathology & Medicine, Wiley, Vol. 44, No. 5 ( 2015-05), p. 367-377
Abstract:
Keratocystic odontogenic tumour ( KCOT ) is a benign, yet aggressive odontogenic tumour. Herein, proteome analysis of KCOT lesions in comparison with control patient‐matched tissue unaffected by the disease and with inflammatory odontogenic cysts, namely radicular cysts is presented. Methods For the proteomics profiling, two complementary proteomics techniques MALDI ‐ MS / MS and LC ‐ ESI ‐ MS / MS were employed. Potential candidate biomarkers were validated by immunohistochemistry. Results More than 43 proteins were found to be differentially expressed or up‐regulated in KCOT lesions in comparison with patient‐matched unaffected oral mucosa. These proteins bear important biological functions and are involved in cell proliferation, cytoskeletal re‐organization, transcription, cellular motility and apoptosis. In particular, a number of differentially expressed proteins participate in autocrine regulation and signalization within JNK and p38 MAPK signalling pathways. Conclusions Immunohistochemical validation of chosen putative biomarkers revealed axin interaction partner and dorsalization‐antagonist ( AIDA ), known as a protein that blocks activation of JNK signalling pathway, as a differential biomarker for KCOT lesions on an independent cohort of KCOT tissue samples in comparison with most prevalent intra‐oseal lesions inflammatory odontogenic cysts.
Type of Medium:
Online Resource
ISSN:
0904-2512
,
1600-0714
DOI:
10.1111/jop.2015.44.issue-5
Language:
English
Publisher:
Wiley
Publication Date:
2015
detail.hit.zdb_id:
2026385-5
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