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  • 1
    Online Resource
    Online Resource
    Mesenchymal Stromal Cell–Derived Small Extracellular Vesicles Induce Ischemic Neuroprotection by Modulating Leukocytes and Specifically Neutrophils
    Ovid Technologies (Wolters Kluwer Health) ; 2020
    In:  Stroke Vol. 51, No. 6 ( 2020-06), p. 1825-1834
    Details
    In: Stroke, Ovid Technologies (Wolters Kluwer Health), Vol. 51, No. 6 ( 2020-06), p. 1825-1834
    Abstract: Small extracellular vesicles (sEVs) obtained from mesenchymal stromal cells (MSCs) were shown to induce neurological recovery after focal cerebral ischemia in rodents and to reverse postischemic lymphopenia in peripheral blood. Since peripheral blood cells, especially polymorphonuclear neutrophils (PMNs), contribute to ischemic brain injury, we analyzed brain leukocyte responses to sEVs and investigated the role of PMNs in sEV-induced neuroprotection. Methods— Male C57Bl6/j mice were exposed to transient intraluminal middle cerebral artery occlusion. After reperfusion, vehicle or sEVs prepared from conditioned media of MSCs raised from bone marrow samples of 3 randomly selected healthy human donors were intravenously administered. sEVs obtained from normoxic and hypoxic MSCs were applied. PMNs were depleted in vehicle and MSC-sEV–treated mice. Neurological deficits, ischemic injury, blood-brain barrier integrity, peripheral blood leukocyte responses, and brain leukocyte infiltration were evaluated over 72 hours. Results— sEV preparations of all 3 donors collected from normoxic MSCs significantly reduced neurological deficits. Preparations of 2 of these donors significantly decreased infarct volume and neuronal injury. sEV-induced neuroprotection was consistently associated with a decreased brain infiltration of leukocytes, namely of PMNs, monocytes/macrophages, and lymphocytes. sEVs obtained from hypoxic MSCs (1% O 2 ) had similar effects on neurological deficits and ischemic injury as MSC-sEVs obtained under regular conditions (21% O 2 ) but also reduced serum IgG extravasation—a marker of blood-brain barrier permeability. PMN depletion mimicked the effects of MSC-sEVs on neurological recovery, ischemic injury, and brain PMN, monocyte, and lymphocyte counts. Combined MSC-sEV administration and PMN depletion did not have any effects superior to PMN depletion in any of the readouts examined. Conclusions— Leukocytes and specifically PMNs contribute to MSC-sEV–induced ischemic neuroprotection. Individual MSC-sEV preparations may differ in their neuroprotective activities. Potency assays are urgently needed to identify their therapeutic efficacy before clinical application. Visual Overview— An online visual overview is available for this article.
    Type of Medium: Online Resource
    ISSN: 0039-2499 , 1524-4628
    URL: Article
    DOI: 10.1161/STROKEAHA.119.028012
    RVK:
    XA 10000
    Language: English
    Publisher: Ovid Technologies (Wolters Kluwer Health)
    Publication Date: 2020
    detail.hit.zdb_id: 1467823-8
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  • 2
    Online Resource
    Online Resource
    Questioning on Pers Role Toward Publik Interests and The Need of Media Literacy
    Universitas Mataram ; 2022
    In:  Proceedings Of International Conference On Communication Science Vol. 2, No. 1 ( 2022-11-10), p. 95-103
    Details
    In: Proceedings Of International Conference On Communication Science, Universitas Mataram, Vol. 2, No. 1 ( 2022-11-10), p. 95-103
    Abstract: The relationship that occurs between the media and the public does not always lead to a symbiotic mutualism. The media and the public in some relations give rise to tug of war interests, sometimes they are balanced, sometimes they overlap. Ideally, the media position themselves as providers of content that are proportionate to the public. Proportional here in the sense of according to what is needed by the public. However, in practice, the political economy of the media is an unavoidable problem because the relationship between the media and the public also involves industry as the environment in which the media is located. When industrialization is attached to the media, the relationship between the media and the public is very likely to run unequally. The public is no longer positioned as accessing information that must be served and considered. In fact, the public is placed as a commodity whose interests in the media seem to have been neglected. As a result, there is a lot of media content that overrides the public interest because it is merely pursuing capital. From this point, the demand for the public to be active and critical becomes important. In the end, the media literacy movement is no longer a made-up demand, but a necessity so that the public, as the owner of resources, has their interests protected.
    Type of Medium: Online Resource
    ISSN: 2829-3231
    URL: Issue
    URL: Article
    DOI: 10.29303/iccsproceeding.v2i1
    DOI: 10.29303/iccsproceeding.v2i1.57
    Language: Unknown
    Publisher: Universitas Mataram
    Publication Date: 2022
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  • 3
    Online Resource
    Online Resource
    Patients with SARS-CoV-2-Induced Viral Sepsis Simultaneously Show Immune Activation, Impaired Immune Function and a Procoagulatory Disease State
    MDPI AG ; 2023
    In:  Vaccines Vol. 11, No. 2 ( 2023-02-13), p. 435-
    Details
    In: Vaccines, MDPI AG, Vol. 11, No. 2 ( 2023-02-13), p. 435-
    Abstract: Background: It is widely accepted that SARS-CoV-2 causes a dysregulation of immune and coagulation processes. In severely affected patients, viral sepsis may result in life endangering multiple organ dysfunction. Furthermore, most therapies for COVID-19 patients target either the immune system or coagulation processes. As the exact mechanism causing SARS-CoV-2-induced morbidity and mortality was unknown, we started an in-depth analysis of immunologic and coagulation processes. Methods: 127 COVID-19 patients were treated at the University Hospital Essen, Germany, between May 2020 and February 2022. Patients were divided according to their maximum COVID-19 WHO ordinal severity score (WHO 0–10) into hospitalized patients with a non-severe course of disease (WHO 4–5, n = 52) and those with a severe course of disease (WHO 6–10, n = 75). Non-infected individuals served as healthy controls (WHO 0, n = 42). Blood was analyzed with respect to cell numbers, clotting factors, as well as pro- and anti-inflammatory mediators in plasma. As functional parameters, phagocytosis and inflammatory responses to LPS and antigen-specific stimulation were determined in monocytes, granulocytes, and T cells using flow cytometry. Findings: In the present study, immune and coagulation systems were analyzed simultaneously. Interestingly, many severe COVID-19 patients showed an upregulation of pro-inflammatory mediators and at the same time clear signs of immunosuppression. Furthermore, severe COVID-19 patients not only exhibited a disturbed immune system, but in addition showed a pronounced pro-coagulation phenotype with impaired fibrinolysis. Therefore, our study adds another puzzle piece to the already complex picture of COVID-19 pathology implying that therapies in COVID-19 must be individualized. Conclusion: Despite years of research, COVID-19 has not been understood completely and still no therapies exist, fitting all requirements and phases of COVID-19 disease. This observation is highly reminiscent to sepsis. Research in sepsis has been going on for decades, while the disease is still not completely understood and therapies fitting all patients are lacking as well. In both septic and COVID-19 patients, immune activation can be accompanied by immune paralysis, complicating therapeutic intervention. Accordingly, therapies that lower immune activation may cause detrimental effects in patients, who are immune paralyzed by viral infections or sepsis. We therefore suggest individualizing therapies and to broaden the spectrum of immunological parameters analyzed before therapy. Only if the immune status of a patient is understood, can a therapeutic intervention be successful.
    Type of Medium: Online Resource
    ISSN: 2076-393X
    URL: Article
    DOI: 10.3390/vaccines11020435
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2703319-3
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  • 4
    Online Resource
    Online Resource
    Berita korupsi yang elitis: studi wacana kritis atas berita korupsi untuk membongkar praktik pemberitaan yang elitis oleh media massa cetak
    Indonesian Institute for Counseling, Education and Therapy ; 2023
    In:  JPPI (Jurnal Penelitian Pendidikan Indonesia) Vol. 9, No. 1 ( 2023-03-31), p. 319-
    Details
    In: JPPI (Jurnal Penelitian Pendidikan Indonesia), Indonesian Institute for Counseling, Education and Therapy, Vol. 9, No. 1 ( 2023-03-31), p. 319-
    Abstract: 〈 span lang="EN-US" 〉 Surat kabar di Provinsi Nusa Tenggara Timur (NTT) memproduksi dan mengontruksi sejumlah teks berita korupsi. Penelitian ini hendak mencermati dengan perspektif kritis wacana korupsi tersebut. Peneliti menggunakan analisis wacana kritis (Critical Discourse Analysis/CDA) yang ditawarkan oleh Teun A. van Dijk untuk menganalisis teks, kognisi sosial dan konteks sosial. Data teks berita korupsi dihimpun peneliti dari ketiga media massa lokal tersebut, sedangkan data kognisi sosial diperoleh melalui wawancara dengan wartawan yang menulis berita korupsi serta data konteks sosial dihimpun dari sebuah LSM yang concern dengan masalah korupsi di NTT yakni PIAR-NTT serta melalui observasi lapangan. Peneliti menganalisis ketiga elemen wacana yakni teks, kognisi sosial, dan konteks sosial. Temuan dari penelitian ini adalah berita korupsi dari surat kabar di NTT adalah adanya faktor yang turut mempengaruhi terbentuknya teks berita korupsi menjadi berita yang elitis seperti kepemilikan media, relasi kuasa dari aparat penegak hukum dan pejabat daerah. Media massa cetak dapat terkooptasi oleh kepentingan pemilik modal, pasar atau negara. Konsep van Dijk tentang media yang tidak netral diafirmasikan kembali di dalam penelitian ini. Sikap kritis tentu sangat dibutuhkan oleh siapa saja yang membaca berita pada media massa. 〈 /span 〉
    Type of Medium: Online Resource
    ISSN: 2502-8103 , 2477-8524
    URL: Issue
    URL: Article
    DOI: 10.29210/jppi.v9i1
    DOI: 10.29210/020231697
    Language: Unknown
    Publisher: Indonesian Institute for Counseling, Education and Therapy
    Publication Date: 2023
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  • 5
    Online Resource
    Online Resource
    Small extracellular vesicles obtained from hypoxic mesenchymal stromal cells have unique characteristics that promote cerebral angiogenesis, brain remodeling and neurological recovery after focal cerebral ischemia in mice
    Springer Science and Business Media LLC ; 2021
    In:  Basic Research in Cardiology Vol. 116, No. 1 ( 2021-12)
    Details
    In: Basic Research in Cardiology, Springer Science and Business Media LLC, Vol. 116, No. 1 ( 2021-12)
    Abstract: Obtained from the right cell-type, mesenchymal stromal cell (MSC)-derived small extracellular vesicles (sEVs) promote stroke recovery. Within this process, microvascular remodeling plays a central role. Herein, we evaluated the effects of MSC-sEVs on the proliferation, migration, and tube formation of human cerebral microvascular endothelial cells (hCMEC/D3) in vitro and on post-ischemic angiogenesis, brain remodeling and neurological recovery after middle cerebral artery occlusion (MCAO) in mice. In vitro, sEVs obtained from hypoxic (1% O 2 ), but not ‘normoxic’ (21% O 2 ) MSCs dose-dependently promoted endothelial proliferation, migration, and tube formation and increased post-ischemic endothelial survival. sEVs from hypoxic MSCs regulated a distinct set of miRNAs in hCMEC/D3 cells previously linked to angiogenesis, three being upregulated (miR-126-3p, miR-140-5p, let-7c-5p) and three downregulated (miR-186-5p, miR-370-3p, miR-409-3p). LC/MS–MS revealed 52 proteins differentially abundant in sEVs from hypoxic and ‘normoxic’ MSCs. 19 proteins were enriched (among them proteins involved in extracellular matrix–receptor interaction, focal adhesion, leukocyte transendothelial migration, protein digestion, and absorption), and 33 proteins reduced (among them proteins associated with metabolic pathways, extracellular matrix–receptor interaction, focal adhesion, and actin cytoskeleton) in hypoxic MSC-sEVs. Post-MCAO, sEVs from hypoxic MSCs increased microvascular length and branching point density in previously ischemic tissue assessed by 3D light sheet microscopy over up to 56 days, reduced delayed neuronal degeneration and brain atrophy, and enhanced neurological recovery. sEV-induced angiogenesis in vivo depended on the presence of polymorphonuclear neutrophils. In neutrophil-depleted mice, MSC-sEVs did not influence microvascular remodeling. sEVs from hypoxic MSCs have distinct angiogenic properties. Hypoxic preconditioning enhances the restorative effects of MSC-sEVs.
    Type of Medium: Online Resource
    ISSN: 0300-8428 , 1435-1803
    URL: Article
    DOI: 10.1007/s00395-021-00881-9
    RVK:
    XA 31125
    RVK:
    XA 10000
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 1458470-0
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  • 6
    Online Resource
    Online Resource
    Pathophysiologie der Sepsis
    Georg Thieme Verlag KG ; 2023
    In:  Intensivmedizin up2date Vol. 19, No. 03 ( 2023-09), p. 257-273
    Details
    In: Intensivmedizin up2date, Georg Thieme Verlag KG, Vol. 19, No. 03 ( 2023-09), p. 257-273
    Type of Medium: Online Resource
    ISSN: 1614-4856 , 1614-6697
    URL: Article
    DOI: 10.1055/a-2114-8333
    Language: German
    Publisher: Georg Thieme Verlag KG
    Publication Date: 2023
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  • 7
    Online Resource
    Online Resource
    Upregulation of miRNA-200c during Disease Progression in COVID-19 Patients
    MDPI AG ; 2022
    In:  Journal of Clinical Medicine Vol. 12, No. 1 ( 2022-12-29), p. 283-
    Details
    In: Journal of Clinical Medicine, MDPI AG, Vol. 12, No. 1 ( 2022-12-29), p. 283-
    Abstract: The COVID-19 pandemic has caused more than 6 million deaths worldwide since its first outbreak in December 2019 and continues to be a major health problem. Several studies have established that the infection by SARS-CoV-2 can be categorized in a viremic, acute and recovery or severe phase. Hyperinflammation during the acute pneumonia phase is a major cause of severe disease progression and death. Treatment of COVID-19 with directly acting antivirals is limited within a narrow window of time between first clinical symptoms and the hyperinflammatory response. Therefore, early initiation of treatment is crucial to assure optimal health care for patients. Molecular diagnostic biomarkers represent a potent tool to predict the course of disease and thus to assess the optimal treatment regimen and time point. Here, we investigated miRNA-200c as a potential marker for the prediction of the severity of COVID-19 to preventively initiate and personalize therapeutic interventions in the future. We found that miRNA-200c correlates with the severity of disease. With retrospective analysis, however, there is no correlation with prognosis at the time of hospitalization. Our study provides the basis for further evaluation of miRNA-200c as a predictive biomarker for the progress of COVID-19.
    Type of Medium: Online Resource
    ISSN: 2077-0383
    URL: Article
    DOI: 10.3390/jcm12010283
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2662592-1
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  • 8
    Online Resource
    Online Resource
    Role of Local Mass Media Control of Regional Governance in Indonesia (Case Study in East Nusa Tenggara)
    Canadian Center of Science and Education ; 2019
    In:  Asian Social Science Vol. 15, No. 10 ( 2019-09-29), p. 14-
    Details
    In: Asian Social Science, Canadian Center of Science and Education, Vol. 15, No. 10 ( 2019-09-29), p. 14-
    Abstract: Background. Decentralization of the authority to manage government and regional development has been regulated in Law Number 32 of 2014 concerning Regional Government. However, in its implementation there are many deviations found in the form of corruption of local officials causing a loss of quality in human resources. The researcher tried to reveal the role of local mass media to control local governance so that its implementation did not cause corruption in the development budget carried out by regional government officials. Method and material. Using qualitative analysis of news texts published by local mass media with and content of corrupt behavior of regional officials in East Nusa Tenggara Province. Various secondary data is used to supplement this research information. Respondents of journalists and local media editor in chief. Results. Local media coverage revealed 100 regional heads were arrested and convicted of corruption. The impact of the worsening social conditions in the East Nusa Tenggara the high prevalence of stunting is 40.30%; 2,669 infants with malnourished babies, 1,142,790 poor people (21.35%) in 2018. Conclusion. It has been punished by regional officials who are perpetrators of corruption as a result of local media coverage. Corruption has a systemic impact on decreasing the quality of human resources in East Nusa Tenggara.
    Type of Medium: Online Resource
    ISSN: 1911-2025 , 1911-2017
    URL: Article
    DOI: 10.5539/ass.v15n10p14
    Language: Unknown
    Publisher: Canadian Center of Science and Education
    Publication Date: 2019
    detail.hit.zdb_id: 2434887-9
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  • 9
    Online Resource
    Online Resource
    Local language and cultural conflict in Flores Island Society. A Critical Linguistics Study on Cross Cultural Communication in the Middle of Cultural Conflict in Flores Island Society
    PLUS COMMUNICATION CONSULTING SRL ; 2021
    In:  Technium Social Sciences Journal Vol. 21 ( 2021-07-09), p. 431-438
    Details
    In: Technium Social Sciences Journal, PLUS COMMUNICATION CONSULTING SRL, Vol. 21 ( 2021-07-09), p. 431-438
    Abstract: Flores Island is one of the diverse place despite its small size as the part of Indonesian East Archipelago and Lesser Sunda Island. We all know that regional language or local language is an intellectual and cultural property. Local language lives and develops in an area or community. There are many regional languages accustomed to be used as a means of communication. The use of local language with its different meanings in a region or community that is no longer homogeneous can be a trigger to cultural conflict. The causes of conflict among residents or cultural believers are assimilation and mixing of heterogeneous local language-speaking communities. This study aims at revealing the existence of cultural conflicts as a result of the meaning differences of local languagesused by the people on Flores Island. This study uses a critical linguistics approach with a cross-cultural communication paradigm.
    Type of Medium: Online Resource
    ISSN: 2668-7798
    URL: Issue
    URL: Article
    DOI: 10.47577/tssj.v21i1
    DOI: 10.47577/tssj.v21i1.3845
    Language: Unknown
    Publisher: PLUS COMMUNICATION CONSULTING SRL
    Publication Date: 2021
    detail.hit.zdb_id: 3007014-4
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  • 10
    Online Resource
    Online Resource
    KONFLIK BUDAYA LOKAL PADA MASYARAKAT DI PULAU FLORES (SEBUAH ANALISIS KOMUNIKASI LINTAS BUDAYA)
    Universitas Islam Indonesia (Islamic University of Indonesia) ; 2014
    In:  Jurnal Komunikasi Vol. 9, No. 1 ( 2014-10-02), p. 59-68
    Details
    In: Jurnal Komunikasi, Universitas Islam Indonesia (Islamic University of Indonesia), Vol. 9, No. 1 ( 2014-10-02), p. 59-68
    Type of Medium: Online Resource
    ISSN: 1907-848X , 2548-7647
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.20885/komunikasi.vol9.iss1
    DOI: 10.20885/komunikasi
    DOI: 10.20885/komunikasi.vol9.iss1.art5
    Language: Unknown
    Publisher: Universitas Islam Indonesia (Islamic University of Indonesia)
    Publication Date: 2014
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