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  • 1
    Online Resource
    Online Resource
    Cell spreading on quartz crystal microbalance elicits positive frequency shifts indicative of viscosity changes
    Springer Science and Business Media LLC ; 2003
    In:  Analytical and Bioanalytical Chemistry Vol. 377, No. 3 ( 2003-10-1), p. 578-586
    Details
    In: Analytical and Bioanalytical Chemistry, Springer Science and Business Media LLC, Vol. 377, No. 3 ( 2003-10-1), p. 578-586
    Type of Medium: Online Resource
    ISSN: 1618-2642 , 1618-2650
    URL: Article
    DOI: 10.1007/s00216-003-2080-1
    RVK:
    VA 4300
    Language: Unknown
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2003
    detail.hit.zdb_id: 1459122-4
    detail.hit.zdb_id: 2071767-2
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Machine learning for cross-scale microscopy of viruses
    Elsevier BV ; 2023
    In:  Cell Reports Methods Vol. 3, No. 9 ( 2023-09), p. 100557-
    Details
    In: Cell Reports Methods, Elsevier BV, Vol. 3, No. 9 ( 2023-09), p. 100557-
    Type of Medium: Online Resource
    ISSN: 2667-2375
    URL: Article
    DOI: 10.1016/j.crmeth.2023.100557
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 3091714-1
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  • 3
    Online Resource
    Online Resource
    Galactosyltransferase‐dependent sialylation of complex and endo‐ N ‐acetylglucosaminidase H‐treated core N ‐glycans in vitro
    Wiley ; 1986
    In:  FEBS Letters Vol. 203, No. 1 ( 1986-07-14), p. 64-68
    Details
    In: FEBS Letters, Wiley, Vol. 203, No. 1 ( 1986-07-14), p. 64-68
    Abstract: Oligomannose glycan Ovalbumin Endo‐β‐N‐acetylglucosaminidase H 2 Invertase (Saccharomyces cerevisiae) Glycosylation
    Type of Medium: Online Resource
    ISSN: 0014-5793 , 1873-3468
    URL: Article
    DOI: 10.1016/0014-5793(86)81437-5
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 1986
    detail.hit.zdb_id: 1460391-3
    SSG: 12
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  • 4
    Online Resource
    Online Resource
    Two years into COVID‐19 – Lessons in SARS‐CoV‐2 and a perspective from papers in FEBS Letters
    Wiley ; 2021
    In:  FEBS Letters Vol. 595, No. 23 ( 2021-12), p. 2847-2853
    Details
    In: FEBS Letters, Wiley, Vol. 595, No. 23 ( 2021-12), p. 2847-2853
    Abstract: The 2019 outbreak of coronavirus disease (COVID‐19) in Wuhan (Hubei province of China) has given rise to a pandemic spread of virus, more than 240 million incidences and a death toll larger than 5 million people. COVID‐19 has set off large efforts in research, therapy and patient care, as well as public and private debates in every imaginable form. A number of scientists used the publication platforms provided by the Federation of the European Biochemical Societies (FEBS) to present their research data, reviews, opinions and other contributions relating to COVID‐19 and severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Here, I highlight the recent COVID‐19 papers which have been published and collected in a Virtual Issue in FEBS Letters , and discuss their implications towards understanding the molecular, biochemical and cellular mechanisms of SARS‐CoV‐2 infections, vaccine development and antiviral discovery strategies.
    Type of Medium: Online Resource
    ISSN: 0014-5793 , 1873-3468
    URL: Issue
    URL: Article
    DOI: 10.1002/feb2.v595.23
    DOI: 10.1002/1873-3468.14226
    RVK:
    WA 15000
    Language: English
    Publisher: Wiley
    Publication Date: 2021
    detail.hit.zdb_id: 1460391-3
    SSG: 12
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  • 5
    Online Resource
    Online Resource
    Adenovirus endocytosis
    Wiley ; 2003
    In:  The Journal of Gene Medicine Vol. 5, No. 6 ( 2003-06), p. 451-462
    Details
    In: The Journal of Gene Medicine, Wiley, Vol. 5, No. 6 ( 2003-06), p. 451-462
    Type of Medium: Online Resource
    ISSN: 1099-498X , 1521-2254
    URL: Issue
    URL: Journal
    URL: Article
    DOI: 10.1002/jgm.v5:6
    DOI: 10.1002/(ISSN)1521-2254
    DOI: 10.1002/jgm.409
    Language: English
    Publisher: Wiley
    Publication Date: 2003
    detail.hit.zdb_id: 2002203-7
    SSG: 12
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  • 6
    Online Resource
    Online Resource
    Host requirements for infectious virus entry
    Elsevier BV ; 2007
    In:  GBM Fall meeting Hamburg 2007 Vol. 2007, No. Fall ( 2007-9)
    Details
    In: GBM Fall meeting Hamburg 2007, Elsevier BV, Vol. 2007, No. Fall ( 2007-9)
    Type of Medium: Online Resource
    URL: Article
    DOI: 10.1240/sav_gbm_2007_h_002067
    Language: Unknown
    Publisher: Elsevier BV
    Publication Date: 2007
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  • 7
    Online Resource
    Online Resource
    Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro
    Springer Science and Business Media LLC ; 2010
    In:  Virology Journal Vol. 7, No. 1 ( 2010-12)
    Details
    In: Virology Journal, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2010-12)
    Abstract: Human adenoviruses (Ads) have substantial potential for clinical applications in cancer patients. Conditionally replicating adenoviruses (CRAds) include oncolytic adenoviruses in which expression of the immediate early viral transactivator protein E1A is controlled by a cancer cell-selective promoter. To enhance efficacy, CRAds are further armed to contain therapeutic genes. Due to size constraints of the capsid geometry, the capacity for packaging transgenes into Ads is, however, limited. To overcome this limitation, the employment of E1A-deleted replication-deficient viruses carrying therapeutic genes in combination with replication-competent CRAd vectors expressing E1A in trans has been proposed. Most trans-complementing studies involved transgene expressions from strong ubiquitous promoters, and thereby relied entirely on the cancer cell specificity of the CRAd vector. Results Here we tested the trans-complementation of a CRAd and a replication-deficient transgene vector containing the same cancer cell-selective promoter. Hereto, we generated two new vectors expressing IL-2 and CD40L from a bicistronic expression cassette under the control of the melanoma/melanocyte-specific tyrosinase enhancer tyrosinase promoter (TETP), which we previously described for the melanoma-specific CRAd vector AdΔEP-TETP. These vectors gave rise to tightly controlled melanoma-specific transgene expression levels, which were only 5 to 40-fold lower than those from vectors controlled by the nonselective CMV promoter. Reporter analyses using Ad-CMV-eGFP in combination with AdΔEP-TETP revealed a high level of trans-complementation in melanoma cells (up to about 30-fold), but not in non-melanoma cells, unlike the AdCMV-eGFP/wtAd5 binary vector system, which was equally efficient in melanoma and non-melanoma cells. Similar findings were obtained when replacing the transgene vector AdCMV-eGFP with AdCMV-IL-2 or AdCMV-CD40L. However, the combination of the novel AdTETP-CD40L/IL-2 vector with AdΔEP-TETP or wtAd5 gave reproducible moderate 3-fold enhancements of IL-2 by trans-complementation only. Conclusions The cancer cell-selective TETP tested here did not give the expected enforceable transgene expression typically achieved in the Ad trans-complementing system. Reasons for this could include virus-mediated down regulation of limiting transcription factors, and/or competition for such factors by different promoters. Whether this finding is unique to the particular promoter system tested here, or also occurs with other promoters warrants further investigations.
    Type of Medium: Online Resource
    ISSN: 1743-422X
    URL: Article
    DOI: 10.1186/1743-422X-7-175
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2010
    detail.hit.zdb_id: 2160640-7
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  • 8
    Online Resource
    Online Resource
    Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1
    American Society for Microbiology ; 2017
    In:  Journal of Virology Vol. 91, No. 15 ( 2017-08)
    Details
    In: Journal of Virology, American Society for Microbiology, Vol. 91, No. 15 ( 2017-08)
    Abstract: Adeno-associated virus 2 (AAV2) depends on the simultaneous presence of a helper virus such as herpes simplex virus 1 (HSV-1) for productive replication. At the same time, AAV2 efficiently blocks the replication of HSV-1, which would eventually limit its own replication by diminishing the helper virus reservoir. This discrepancy begs the question of how AAV2 and HSV-1 can coexist in a cell population. Here we show that in coinfected cultures, AAV2 DNA replication takes place almost exclusively in S/G 2 -phase cells, while HSV-1 DNA replication is restricted to G 1 phase. Live microscopy revealed that not only wild-type AAV2 (wtAAV2) replication but also reporter gene expression from both single-stranded and double-stranded (self-complementary) recombinant AAV2 vectors preferentially occurs in S/G 2 -phase cells, suggesting that the preference for S/G 2 phase is independent of the nature of the viral genome. Interestingly, however, a substantial proportion of S/G 2 -phase cells transduced by the double-stranded but not the single-stranded recombinant AAV2 vectors progressed through mitosis in the absence of the helper virus. We conclude that cell cycle-dependent AAV2 rep expression facilitates cell cycle-dependent AAV2 DNA replication and inhibits HSV-1 DNA replication. This may limit competition for cellular and viral helper factors and, hence, creates a biological niche for either virus to replicate. IMPORTANCE Adeno-associated virus 2 (AAV2) differs from most other viruses, as it requires not only a host cell for replication but also a helper virus such as an adenovirus or a herpesvirus. This situation inevitably leads to competition for cellular resources. AAV2 has been shown to efficiently inhibit the replication of helper viruses. Here we present a new facet of the interaction between AAV2 and one of its helper viruses, herpes simplex virus 1 (HSV-1). We observed that AAV2 rep gene expression is cell cycle dependent and gives rise to distinct time-controlled windows for HSV-1 replication. High Rep protein levels in S/G 2 phase support AAV2 replication and inhibit HSV-1 replication. Conversely, low Rep protein levels in G 1 phase permit HSV-1 replication but are insufficient for AAV2 replication. This allows both viruses to productively replicate in distinct sets of dividing cells.
    Type of Medium: Online Resource
    ISSN: 0022-538X , 1098-5514
    URL: Article
    DOI: 10.1128/JVI.00357-17
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2017
    detail.hit.zdb_id: 1495529-5
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  • 9
    Online Resource
    Online Resource
    Cell-to-cell and genome-to-genome variability of adenovirus transcription tuned by the cell cycle
    The Company of Biologists ; 2021
    In:  Journal of Cell Science Vol. 134, No. 5 ( 2021-03-01)
    Details
    In: Journal of Cell Science, The Company of Biologists, Vol. 134, No. 5 ( 2021-03-01)
    Abstract: In clonal cultures, not all cells are equally susceptible to virus infection, and the mechanisms underlying this are poorly understood. Here, we developed image-based single-cell measurements to scrutinize the heterogeneity of adenovirus (AdV) infection. AdV delivers, transcribes and replicates a linear double-stranded DNA genome in the nucleus. We measured the abundance of viral transcripts using single-molecule RNA fluorescence in situ hybridization (FISH) and the incoming 5-ethynyl-2′-deoxycytidine (EdC)-tagged viral genomes using a copper(I)-catalyzed azide–alkyne cycloaddition (click) reaction. Surprisingly, expression of the immediate early gene E1A only moderately correlated with the number of viral genomes in the cell nucleus. Intranuclear genome-to-genome heterogeneity was found at the level of viral transcription and, in accordance, individual genomes exhibited heterogeneous replication activity. By analyzing the cell cycle state, we found that G1 cells exhibited the highest E1A gene expression and displayed increased correlation between E1A gene expression and viral genome copy numbers. The combined image-based single-molecule procedures described here are ideally suited to explore the cell-to-cell variability in viral gene expression in a range of different settings, including the innate immune response. This article has an associated First Person interview with the first author of the paper.
    Type of Medium: Online Resource
    ISSN: 0021-9533 , 1477-9137
    URL: Article
    DOI: 10.1242/jcs.252544
    Language: English
    Publisher: The Company of Biologists
    Publication Date: 2021
    detail.hit.zdb_id: 219171-4
    detail.hit.zdb_id: 1483099-1
    SSG: 12
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  • 10
    Online Resource
    Online Resource
    The FDA-Approved Drug Nelfinavir Inhibits Lytic Cell-Free but Not Cell-Associated Nonlytic Transmission of Human Adenovirus
    American Society for Microbiology ; 2020
    In:  Antimicrobial Agents and Chemotherapy Vol. 64, No. 9 ( 2020-08-20)
    Details
    In: Antimicrobial Agents and Chemotherapy, American Society for Microbiology, Vol. 64, No. 9 ( 2020-08-20)
    Abstract: Adenoviruses (AdVs) are prevalent and give rise to chronic and recurrent disease. Human AdV (HAdV) species B and C, such as HAdV-C2, -C5, and -B14, cause respiratory disease and constitute a health threat for immunocompromised individuals. HAdV-Cs are well known for lysing cells owing to the E3 CR1-β-encoded adenovirus death protein (ADP). We previously reported a high-throughput image-based screening framework and identified an inhibitor of HAdV-C2 multiround infection, nelfinavir mesylate. Nelfinavir is the active ingredient of Viracept, an FDA-approved inhibitor of human immunodeficiency virus (HIV) aspartyl protease that is used to treat AIDS. It is not effective against single-round HAdV infections. Here, we show that nelfinavir inhibits lytic cell-free transmission of HAdV, indicated by the suppression of comet-shaped infection foci in cell culture. Comet-shaped foci occur upon convection-based transmission of cell-free viral particles from an infected cell to neighboring uninfected cells. HAdV lacking ADP was insensitive to nelfinavir but gave rise to comet-shaped foci, indicating that ADP enhances but is not required for cell lysis. This was supported by the notion that HAdV-B14 and -B14p1 lacking ADP were highly sensitive to nelfinavir, although HAdV-A31, -B3, -B7, -B11, -B16, -B21, -D8, -D30, and -D37 were less sensitive. Conspicuously, nelfinavir uncovered slow-growing round HAdV-C2 foci, independent of neutralizing antibodies in the medium, indicative of nonlytic cell-to-cell transmission. Our study demonstrates the repurposing potential of nelfinavir with postexposure efficacy against different HAdVs and describes an alternative nonlytic cell-to-cell transmission mode of HAdV.
    Type of Medium: Online Resource
    ISSN: 0066-4804 , 1098-6596
    URL: Article
    DOI: 10.1128/AAC.01002-20
    RVK:
    XA 24552
    Language: English
    Publisher: American Society for Microbiology
    Publication Date: 2020
    detail.hit.zdb_id: 1496156-8
    SSG: 12
    SSG: 15,3
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