In:
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, Vol. 72, No. 5 ( 2022-05-27)
Abstract:
Two strains designated as c1 T and c7 T , were isolated from the landfill leachate of a domestic waste treatment plant in Huizhou City, Guangdong Province, PR China. The cells of both strains were aerobic, rod-shaped, non-motile and formed yellow colonies on Reasoner’s 2A agar plates. Strain c1 T grew at 10–42 °C (optimum, 30 °C), pH 4.5–10.5 (optimum, pH 7.0) and 0–2.0 % (w/v) NaCl (optimum, 0–0.5 %). Strain c7 T grew at 10–42 °C (optimum, 30 °C), pH 4.5–10.5 (optimum, pH 6.0) and 0–2.0 % (w/v) NaCl (optimum, 0–0.5 %). Phylogenetic analyses revealed that strains c1 T and c7 T belong to the genus Novosphingobium . The 16S rRNA gene sequence similarities of strains c1 T and c7 T to the type strains of Novosphingobium species were 94.5–98.2 % and 94.3–99.1 %, respectively. The calculated pairwise average nucleotide identity values among strains c1 T , c7 T and the reference strains were in the range of 75.2–85.9 % and the calculated pairwise average amino acid identity values among strains c1 T , c7 T and reference strains were in the range of 72.0–88.3 %. Their major respiratory quinone was Q-10, and the major cellular fatty acids were C 18 : 1 ω 7 c , C 18 : 0 , C 16 : 1 ω 7 c , C 16 : 0 and C 14 : 0 2OH. The major polar lipids of strains c1 T and c7 T were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, sphingoglycolipid, unidentified lipids and unidentified phospholipid. Based on phenotypic, chemotaxonomic, phylogenetic and genomic results from this study, strains c1 T and c7 T should represent two independent novel species of Novosphingobium , for which the names Novosphingobium percolationis sp. nov. (type strain c1 T =GDMCC 1.2555 T =KCTC 82826 T ) and Novosphingobium huizhouense sp. nov. (type strain c7 T =GDMCC 1.2556 T =KCTC 82827 T ) are proposed. The gene function annotation results of strains c1 T and c7 T suggest that they could play an important role in the degradation of organic pollutants.
Type of Medium:
Online Resource
ISSN:
1466-5026
,
1466-5034
DOI:
10.1099/ijsem.0.005394
Language:
English
Publisher:
Microbiology Society
Publication Date:
2022
detail.hit.zdb_id:
215062-1
detail.hit.zdb_id:
2056611-6
SSG:
12
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