GLORIA

GEOMAR Library Ocean Research Information Access

X

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Online Resource
    Online Resource
    CUL4A overexpression as an independent adverse prognosticator in intrahepatic cholangiocarcinoma
    Springer Science and Business Media LLC ; 2017
    In:  BMC Cancer Vol. 17, No. 1 ( 2017-12)
    Details
    In: BMC Cancer, Springer Science and Business Media LLC, Vol. 17, No. 1 ( 2017-12)
    Type of Medium: Online Resource
    ISSN: 1471-2407
    URL: Article
    DOI: 10.1186/s12885-017-3389-z
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2041352-X
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 2
    Online Resource
    Online Resource
    Development of a nanobody-based immunoassay for the sensitive detection of fibrinogen-like protein 1
    Springer Science and Business Media LLC ; 2021
    In:  Acta Pharmacologica Sinica Vol. 42, No. 11 ( 2021-11), p. 1921-1929
    Details
    In: Acta Pharmacologica Sinica, Springer Science and Business Media LLC, Vol. 42, No. 11 ( 2021-11), p. 1921-1929
    Abstract: Immune checkpoint inhibition is an important strategy in cancer therapy. Blockade of CTLA-4 and PD-1/PD-L1 is well developed in clinical practice. In the last few years, LAG-3 has received much interest as an emerging novel target in immunotherapy. It was recently reported that FGL1 is a major ligand of LAG-3, which is normally secreted by the liver but is upregulated in several human cancers. FGL1 is a crucial biomarker and target for cancer immunotherapy. As the efficacy of immunotherapy is limited to specific types of patients, the subset of patients needs to be selected appropriately to receive precise treatment according to different biomarkers. To date, there is no test to accurately assess FGL1 expression levels. Nanobodies have some outstanding features, such as high stability, solubility and affinity for diagnostic and therapeutic applications. Here, we report the development and validation of a rapid, sensitive, and cost-effective nanobody-based immunoassay for the detection of FGL1 in human serum. In this study, human FGL1 recombinant protein was expressed and purified for the first time as an immunized antigen. Then, we constructed a nanobody phage display library and screened several nanobodies that bind FGL1 with high affinity. We selected two nanobodies targeting different epitopes of FGL1, one as a capture and the other conjugated with HRP as a probe. The double nanobody-based sandwich ELISA to detect the concentration of FGL1 showed a good response relationship in the range of 15.625–2000 ng/mL, and the recoveries from the spiked sample were in the range of 78% and 100%. This assay could be used as a potential approach for evaluating FGL1 expression for patient stratification and for predicting the therapeutic efficacy of targeting the LAG3/FGL1 axis.
    Type of Medium: Online Resource
    ISSN: 1671-4083 , 1745-7254
    URL: Article
    DOI: 10.1038/s41401-020-00574-4
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2021
    detail.hit.zdb_id: 2088565-9
    SSG: 15,3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 3
    Online Resource
    Online Resource
    Photoswitching storage of guest molecules in metal–organic framework for photoswitchable catalysis: exceptional product, ultrahigh photocontrol, and photomodulated size selectivity
    Royal Society of Chemistry (RSC) ; 2017
    In:  Journal of Materials Chemistry A Vol. 5, No. 17 ( 2017), p. 7961-7967
    Details
    In: Journal of Materials Chemistry A, Royal Society of Chemistry (RSC), Vol. 5, No. 17 ( 2017), p. 7961-7967
    Type of Medium: Online Resource
    ISSN: 2050-7488 , 2050-7496
    URL: Article
    DOI: 10.1039/C7TA01388D
    Language: English
    Publisher: Royal Society of Chemistry (RSC)
    Publication Date: 2017
    detail.hit.zdb_id: 2702232-8
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 4
    Online Resource
    Online Resource
    Effects of Residual Stress on Bearing Capacity of Cold-Formed Thin-Walled Steel Members with “л” Section under Axial Compressive Force
    Trans Tech Publications, Ltd. ; 2011
    In:  Advanced Materials Research Vol. 243-249 ( 2011-5), p. 1195-1198
    Details
    In: Advanced Materials Research, Trans Tech Publications, Ltd., Vol. 243-249 ( 2011-5), p. 1195-1198
    Abstract: In order to enhance the pertinence of agriculture building design, the experiment and numerical simulation on cold-formed thin-walled steel members with “л” section under axial compressive force were conducted to investigate the effects of residual stress on bearing capacity of them. For the members, there are inevitably initial defects, such as initial bending, initial eccentricity and residual stress due to the manufacturing error. In the numerical simulation, initial bending and initial eccentricity of the members were considered. The results show that the residual stress affects instability forms of the members. And as the member slenderness ratio increases from 69 to 200, the bearing capacity of member with residual stress decreases from 12.8% to 84.9% of that without residual stress.
    Type of Medium: Online Resource
    ISSN: 1662-8985
    URL: Issue
    URL: Article
    DOI: 10.4028/www.scientific.net/AMR.243-249
    DOI: 10.4028/www.scientific.net/AMR.243-249.1195
    Language: Unknown
    Publisher: Trans Tech Publications, Ltd.
    Publication Date: 2011
    detail.hit.zdb_id: 2265002-7
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 5
    Online Resource
    Online Resource
    Endothelial dysfunction and parenchymal hematoma in ischemic stroke patients after endovascular thrombectomy
    S. Karger AG ; 2023
    In:  Cerebrovascular Diseases
    Details
    In: Cerebrovascular Diseases, S. Karger AG
    Abstract: Background and Purpose: Endothelial dysfunction (ED) may result in parenchymal injury and, therefore, worsen the outcomes of ischemic stroke. This study aimed to determine whether ED could predict parenchymal hematoma (PH) in ischemic stroke patients treated with endovascular thrombectomy (EVT). Methods: Patients with large artery occlusion in the anterior circulation and treated with EVT were prospectively enrolled from 2 stroke centers. Serum soluble intercellular adhesion molecule-1, soluble vascular cell adhesion molecule-1, soluble E-selectin, and von Willebrand factor (vWF) were tested and summed to a standardized score to reflect the levels of ED. PH was diagnosed according to the Heidelberg Bleeding Classification. Results: Of the 325 enrolled patients (mean age, 68.6 years; 207 men), 41 (12.6%) developed PH. Patients with PH had higher concentrations of soluble E-selectin, vWF, and ED sum score. After adjusting for demographic characteristics, National Institutes of Health Stroke Scale score, pre-treatment Alberta stroke program early computerized tomography score, and other potential confounders, the increased ED burden was associated with PH (odds ratio, 1.432; 95% confidence interval, 1.031−1.988; P=0.032). Similar significant results were found in the sensitivity analysis. The multiple-adjusted spline regression model showed a linear association between the total ED score and PH (P=0.001 for linearity). Adding ED score to the conventional model significantly improved the risk prediction of PH (net reclassification improvement=25.2%, P=0.001; integrated discrimination index=2.9%; P=0.001). Conclusions: This study demonstrated that ED might be related to PH. Introducing the ED score could increase the reliability of PH risk model for stroke patients treated with EVT.
    Type of Medium: Online Resource
    ISSN: 1015-9770 , 1421-9786
    URL: Article
    DOI: 10.1159/000530372
    Language: English
    Publisher: S. Karger AG
    Publication Date: 2023
    detail.hit.zdb_id: 1482069-9
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 6
    Online Resource
    Online Resource
    A Nitrogen-Rich Covalent Triazine Framework as a Photocatalyst for Hydrogen Production
    Hindawi Limited ; 2020
    In:  Advances in Polymer Technology Vol. 2020 ( 2020-05-07), p. 1-12
    Details
    In: Advances in Polymer Technology, Hindawi Limited, Vol. 2020 ( 2020-05-07), p. 1-12
    Abstract: Covalent triazine frameworks (CTFs) have emerged as new candidate materials in various research areas such as catalysis, gas separation storage, and energy-related organic devices due to their easy functionalization, high thermal and chemical stability, and permanent porosity. Herein, we report the successful synthesis of a CTF rich in cyano groups (CTF-CN) by the solvothermal condensation of 2,3,6,7-tetrabromonapthalene (TBNDA), Na 2 (1,1-dicyanoethene-2,2-dithiolate), and 1,3,5-tris-(4-aminophenyl)-triazine (TAPT) at 120°C. XRD, SEM, and TEM characterization studies revealed CTF-CN to be amorphous and composed of ultrathin 2D sheets. CTF-CN possessed strong absorption at visible wavelengths, with UV-vis measurements suggesting a band gap energy in the range 2.7-2.9 eV. A 5 wt.% Pt/CTF-CN was found to be a promising photocatalyst for hydrogen production, affording a rate of 487.6  μ mol g -1  h -1 in a H 2 O/TEOA/CH 3 OH solution under visible light. The photocatalytic activity of CTF-CN was benchmarked against g-C 3 N 4 for meaningful assessment of performance. Importantly, the 5 wt.% Pt/CTF-CN photocatalyst exhibited excellent thermal and photocatalytic stability. Further, as a nitrogen-rich porous 2D material, CTF-CN represents a potential platform for the development of novel electrode material for fuel cells and metal ion batteries.
    Type of Medium: Online Resource
    ISSN: 0730-6679 , 1098-2329
    URL: Article
    DOI: 10.1155/2020/7819049
    Language: English
    Publisher: Hindawi Limited
    Publication Date: 2020
    detail.hit.zdb_id: 2014633-4
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 7
    Online Resource
    Online Resource
    Phylogeography Reveals Association between Swine Trade and the Spread of Porcine Epidemic Diarrhea Virus in China and across the World
    Oxford University Press (OUP) ; 2022
    In:  Molecular Biology and Evolution Vol. 39, No. 2 ( 2022-02-03)
    Details
    In: Molecular Biology and Evolution, Oxford University Press (OUP), Vol. 39, No. 2 ( 2022-02-03)
    Abstract: The ongoing SARS (severe acute respiratory syndrome)-CoV (coronavirus)-2 pandemic has exposed major gaps in our knowledge on the origin, ecology, evolution, and spread of animal coronaviruses. Porcine epidemic diarrhea virus (PEDV) is a member of the genus Alphacoronavirus in the family Coronaviridae that may have originated from bats and leads to significant hazards and widespread epidemics in the swine population. The role of local and global trade of live swine and swine-related products in disseminating PEDV remains unclear, especially in developing countries with complex swine production systems. Here, we undertake an in-depth phylogeographic analysis of PEDV sequence data (including 247 newly sequenced samples) and employ an extension of this inference framework that enables formally testing the contribution of a range of predictor variables to the geographic spread of PEDV. Within China, the provinces of Guangdong and Henan were identified as primary hubs for the spread of PEDV, for which we estimate live swine trade to play a very important role. On a global scale, the United States and China maintain the highest number of PEDV lineages. We estimate that, after an initial introduction out of China, the United States acted as an important source of PEDV introductions into Japan, Korea, China, and Mexico. Live swine trade also explains the dispersal of PEDV on a global scale. Given the increasingly global trade of live swine, our findings have important implications for designing prevention and containment measures to combat a wide range of livestock coronaviruses.
    Type of Medium: Online Resource
    ISSN: 0737-4038 , 1537-1719
    URL: Article
    DOI: 10.1093/molbev/msab364
    Language: English
    Publisher: Oxford University Press (OUP)
    Publication Date: 2022
    detail.hit.zdb_id: 2024221-9
    SSG: 12
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 8
    Online Resource
    Online Resource
    DataSheet1.docx
    Frontiers Media SA ; 2023
    In:  Frontiers in Physiology Vol. 14 ( 2023-3-22)
    Details
    In: Frontiers in Physiology, Frontiers Media SA, Vol. 14 ( 2023-3-22)
    Abstract: Introduction: The microbiota–gut–brain axis plays an important role in the pathophysiology of autism spectrum disorder, but its specific mechanisms remain unclear. This study aimed to explore the associations of changes in neurotransmitters and short-chain fatty acids with alterations in gut microbiota in valproic acid model rats. Methods: The autism model rats were established by prenatal exposure to valproic acid (VPA). The Morris water maze test, open field test, and three-chamber test were conducted to assess the behaviors of rats. 16S rRNA gene sequences extracted from fecal samples were used to assess the gut microbial composition. Gas and liquid chromatography–mass spectroscopy was used to identify short-chain fatty acids in fecal samples and neurotransmitters in the prefrontal cortex (PFC). Results: The results showed that 28 bacterial taxa between valproic acid model rats and control rats were identified, and the most differential bacterial taxa in valproic acid model rats and control rats belonged to metagenomic species and Lactobacillus intestinalis . Acetic acid, butyric acid, valeric acid, isobutyric acid, and isovaleric acid were significantly decreased in the valproic acid model rats compared to those in control rats. Five neurotransmitters (threonine, kynurenine, tryptophan, 5-hydroxyindoleacetic acid, denoted as 5-HIAA, and betaine aldehyde chloride, denoted as BAC) were significantly decreased, whereas betaine was increased in the prefrontal cortex of valproic acid model rats compared to control rats. A variety of neurotransmitters (≥4) were correlated with Pseudomonas, Collisella , and Streptococcus at the genus level, and they were also related to the decrease of short-chain fatty acids. Discussion: According to this study, we can preliminarily infer that gut microbiota or their metabolic productions (such as SCFAs) may influence central neurotransmitter metabolism through related pathways of the gut-brain axis. These results provide microbial and short-chain fatty acid (SCFA) frameworks for understanding the role of the microbiota–gut–brain axis in autism spectrum disorder and shed new light on autism spectrum disorder treatment.
    Type of Medium: Online Resource
    ISSN: 1664-042X
    URL: Article
    URL: Article
    DOI: 10.3389/fphys.2023.1077821
    DOI: 10.3389/fphys.2023.1077821.s001
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2564217-0
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 9
    Online Resource
    Online Resource
    Urinary polycyclic aromatic hydrocarbon metabolites as biomarkers of exposure to traffic-emitted pollutants
    Elsevier BV ; 2015
    In:  Environment International Vol. 85 ( 2015-12), p. 104-110
    Details
    In: Environment International, Elsevier BV, Vol. 85 ( 2015-12), p. 104-110
    Type of Medium: Online Resource
    ISSN: 0160-4120
    URL: Article
    DOI: 10.1016/j.envint.2015.09.003
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2015
    detail.hit.zdb_id: 554791-X
    detail.hit.zdb_id: 1497569-5
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
  • 10
    Online Resource
    Online Resource
    Structural and functional characterization of deep-sea thermophilic bacteriophage GVE2 HNH endonuclease
    Springer Science and Business Media LLC ; 2017
    In:  Scientific Reports Vol. 7, No. 1 ( 2017-02-13)
    Details
    In: Scientific Reports, Springer Science and Business Media LLC, Vol. 7, No. 1 ( 2017-02-13)
    Abstract: HNH endonucleases in bacteriophages play a variety of roles in the phage lifecycle as key components of phage DNA packaging machines. The deep-sea thermophilic bacteriophage Geobacillus virus E2 (GVE2) encodes an HNH endonuclease (GVE2 HNHE). Here, the crystal structure of GVE2 HNHE is reported. This is the first structural study of a thermostable HNH endonuclease from a thermophilic bacteriophage. Structural comparison reveals that GVE2 HNHE possesses a typical ββα-metal fold and Zn-finger motif similar to those of HNH endonucleases from other bacteriophages, apart from containing an extra α-helix, suggesting conservation of these enzymes among bacteriophages. Biochemical analysis suggests that the alanine substitutions of the conserved residues (H93, N109 and H118) in the HNH motif of GVE2 HNHE abolished 94%, 60% and 83% of nicking activity, respectively. Compared to the wild type enzyme, the H93A mutant displayed almost the same conformation while the N108A and H118A mutants had different conformations. In addition, the wild type enzyme was more thermostable than the mutants. In the presence of Mn 2+ or Zn 2+ , the wild type enzyme displayed distinct DNA nicking patterns. However, high Mn 2+ concentrations were needed for the N109A and H118A mutants to nick DNA while Zn 2+ inactivated their nicking activity.
    Type of Medium: Online Resource
    ISSN: 2045-2322
    URL: Article
    DOI: 10.1038/srep42542
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2017
    detail.hit.zdb_id: 2615211-3
    Location Call Number Limitation Availability
    BibTip Others were also interested in ...
    Online Resource
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...