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  • 1
    Online Resource
    Online Resource
    Proteomes and Phosphoproteomes of Anther and Pollen: Availability and Progress
    Wiley ; 2017
    In:  PROTEOMICS Vol. 17, No. 20 ( 2017-10)
    Details
    In: PROTEOMICS, Wiley, Vol. 17, No. 20 ( 2017-10)
    Abstract: In flowering plants, anther development plays crucial role in sexual reproduction. Within the anther, microspore mother cells meiosis produces microspores, which further develop into pollen grains that play decisive role in plant reproduction. Previous studies on anther biology mainly focused on single gene functions relying on genetic and molecular methods. Recently, anther development has been expanded from multiple OMICS approaches like transcriptomics, proteomics/phosphoproteomics, and metabolomics. The development of proteomics techniques allowing increased proteome coverage and quantitative measurements of proteins which can characterize proteomes and their modulation during normal development, biotic and abiotic stresses in anther development. In this review, we summarize the achievements of proteomics and phosphoproteomics with anther and pollen organs from model plant and crop species (i.e. Arabidopsis, rice, tobacco). The increased proteomic information facilitated translation of information from the models to crops and thus aid in agricultural improvement.
    Type of Medium: Online Resource
    ISSN: 1615-9853 , 1615-9861
    URL: Issue
    URL: Article
    DOI: 10.1002/pmic.v17.20
    DOI: 10.1002/pmic.201600458
    Language: English
    Publisher: Wiley
    Publication Date: 2017
    detail.hit.zdb_id: 2037674-1
    SSG: 12
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  • 2
    Online Resource
    Online Resource
    Control strategy of ISG hybrid system based on dynamic torque coordination : Control strategy of ISG hybrid system based on dynamic torque coordination
    China Science Publishing & Media Ltd. ; 2013
    In:  JOURNAL OF ELECTRONIC MEASUREMENT AND INSTRUMENT Vol. 27, No. 2 ( 2013-11-29), p. 145-150
    Details
    In: JOURNAL OF ELECTRONIC MEASUREMENT AND INSTRUMENT, China Science Publishing & Media Ltd., Vol. 27, No. 2 ( 2013-11-29), p. 145-150
    Type of Medium: Online Resource
    ISSN: 1000-7105
    URL: Article
    DOI: 10.3724/SP.J.1187.2013.00145
    Language: English
    Publisher: China Science Publishing & Media Ltd.
    Publication Date: 2013
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  • 3
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    Online Resource
    Identification and validation of reference genes for quantitative real-time PCR studies in alligatorweed ( Alternanthera philoxeroides )
    Cambridge University Press (CUP) ; 2021
    In:  Weed Science Vol. 69, No. 4 ( 2021-07), p. 404-411
    Details
    In: Weed Science, Cambridge University Press (CUP), Vol. 69, No. 4 ( 2021-07), p. 404-411
    Abstract: Alligatorweed [ Alternanthera philoxeroides (Mart.) Griseb.] is an invasive semiaquatic weed that poses a serious threat to agricultural production and ecological balance worldwide. However, information about genetic factors associated with the adaptation and invasion mechanisms of this species is limited. Screening for appropriate reference genes is important for gene expression and functional analysis research in A. philoxeroides . In this study, 30 candidate genes that showed stable expression in different A. philoxeroides tissues under various treatments in RNA-seq data were chosen to design quantitative real-time PCR (qRT-PCR) primers. After the amplification specificity validation, 25 candidates were selected and further evaluated in a diverse set of A. philoxeroides samples, including leaf, stem, and root tissues under drought, salinity, heat, chilling, five herbicides, and corresponding untreated controls using qRT-PCR. The delta-CT method, geNorm, NormFinder, BestKeeper, and RefFinder algorithms were used to identify stable reference genes from A. philoxeroides samples. Overall, CoA , RFI2 , Tubby , SRP19 , and V - ATPase were the top five ideal reference genes in all organs and conditions. Tubby and CoA were the most stable reference genes in the leaf/stem; and RFI2 , ERprr , and SPR19 were suitable reference genes for the roots. This work provided a foundation for exploring gene expression profiling of A . philoxeroides , especially those adaptation- and invasion-related genes, which may help in management of this invasive weed.
    Type of Medium: Online Resource
    ISSN: 0043-1745 , 1550-2759
    URL: Article
    DOI: 10.1017/wsc.2021.32
    Language: English
    Publisher: Cambridge University Press (CUP)
    Publication Date: 2021
    detail.hit.zdb_id: 2123881-9
    SSG: 12
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  • 4
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    Hyperglycemia Promotes Endothelial Cell Senescence through AQR/PLAU Signaling Axis
    MDPI AG ; 2022
    In:  International Journal of Molecular Sciences Vol. 23, No. 5 ( 2022-03-07), p. 2879-
    Details
    In: International Journal of Molecular Sciences, MDPI AG, Vol. 23, No. 5 ( 2022-03-07), p. 2879-
    Abstract: Hyperglycemia is reported to accelerate endothelial cell senescence that contributes to diabetic complications. The underlying mechanism, however, remains elusive. We previously demonstrated AQR as a susceptibility gene for type 2 diabetes mellitus (T2DM) and showed that it was increased in multiple tissues in models with T2DM or metabolic syndrome. This study aimed to investigate the role of AQR in hyperglycemia-induced senescence and its underlying mechanism. Here, we retrieved several datasets of the aging models and found the expression of AQR was increased by high glucose and by aging across species, including C. elegans (whole-body), rat (cardiac tissues), and monkey (blood). we validated the increased AQR expression in senescent human umbilical vein endothelial cells (HUVECs). When overexpressed, AQR promoted the endothelial cell senescence, confirmed by an increased number of cells stained with senescence-associated beta-galactosidase and upregulation of CDKN1A (P21) as well as the prohibited cellular colony formation and G2/M phase arrest. To explore the mechanism by which AQR regulated the cellular senescence, transcriptomic analyses of HUVECs with the overexpression and knockdown of the AQR were performed. We identified 52 co-expressed genes that were enriched, in the terms of plasminogen activation, innate immunity, immunity, and antiviral defense. Among co-expressed genes, PLAU was selected to evaluate its contribution to senescence for its highest strength in the enrichment of the biological process. We demonstrated that the knockdown of PLAU rescued senescence-related phenotypes, endothelial cell activation, and inflammation in models induced by AQR or TNF-α. These findings, for the first time, indicate that AQR/PLAU is a critical signaling axis in the modulation of endothelial cell senescence, revealing a novel link between hyperglycemia and vascular dysfunction. The study may have implications in the prevention of premature vascular aging associated with T2DM.
    Type of Medium: Online Resource
    ISSN: 1422-0067
    URL: Article
    DOI: 10.3390/ijms23052879
    Language: English
    Publisher: MDPI AG
    Publication Date: 2022
    detail.hit.zdb_id: 2019364-6
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  • 5
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    Demonstration of phycobilisome mobility by the time- and space-correlated fluorescence imaging of a cyanobacterial cell
    Elsevier BV ; 2007
    In:  Biochimica et Biophysica Acta (BBA) - Bioenergetics Vol. 1767, No. 1 ( 2007-01), p. 15-21
    Details
    In: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Elsevier BV, Vol. 1767, No. 1 ( 2007-01), p. 15-21
    Type of Medium: Online Resource
    ISSN: 0005-2728
    URL: Article
    DOI: 10.1016/j.bbabio.2006.11.012
    RVK:
    WA 15000
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2007
    detail.hit.zdb_id: 2209370-9
    SSG: 12
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  • 6
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    Inside Front Cover: Selective and sensitive Escherichia coli detection based on a T4 bacteriophage‐immobilized multimode microfiber (J. Biophotonics 9/2018)
    Wiley ; 2018
    In:  Journal of Biophotonics Vol. 11, No. 9 ( 2018-09)
    Details
    In: Journal of Biophotonics, Wiley, Vol. 11, No. 9 ( 2018-09)
    Abstract: Sensitive Escherichia coli detection based on a T4 bacteriophageimmobilized multimode microfiber is proposed and demonstrated in this article. Different modes are excited and guided in the microfiber as evanescent field that can interact with surrounding E. coli directly. The change of E. coli concentration and corresponding binding of E. coli on microfiber surface will lead to the shift of optical spectrum, which can be exploited for the application of biosensing. Further details can be found in the article by Yanpeng Li, Hui Ma, Lin Gan, et al. ( e201800012 ). image
    Type of Medium: Online Resource
    ISSN: 1864-063X , 1864-0648
    URL: Issue
    URL: Article
    DOI: 10.1002/jbio.2018.11.issue-9
    DOI: 10.1002/jbio.201870157
    Language: English
    Publisher: Wiley
    Publication Date: 2018
    detail.hit.zdb_id: 2403788-6
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  • 7
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    Dual activity of Serratia marcescens Pt-3 in phosphate-solubilizing and production of antifungal volatiles
    Springer Science and Business Media LLC ; 2022
    In:  BMC Microbiology Vol. 22, No. 1 ( 2022-12)
    Details
    In: BMC Microbiology, Springer Science and Business Media LLC, Vol. 22, No. 1 ( 2022-12)
    Abstract: Soil fertility decline and pathogen infection are severe issues for crop production all over the world. Microbes as inherent factors in soil were effective in alleviating fertility decrease, promoting plant growth and controlling plant pathogens et al. Thus, screening microbes with fertility improving and pathogen controlling properties is of great importance to humans. Results Bacteria Pt-3 isolated from tea rhizosphere showed multiple functions in solubilizing insoluble phosphate, promoting plant growth, producing abundant volatile organic compounds (VOCs) and inhibiting the growth of important fungal pathogens in vitro. According to the 16S rRNA phylogenetic and biochemical analysis, Pt-3 was identified to be Serratia marcescens . The solubilizing zone of Pt-3 in the medium of lecithin and Ca 3 (PO 4 ) 2 was 2.1 cm and 1.8 cm respectively. In liquid medium and soil, the concentration of soluble phosphorus reached 343.9 mg.L − 1 , and 3.98 mg.kg − 1 , and significantly promoted the growth of maize seedling, respectively. Moreover, Pt-3 produced abundant volatiles and greatly inhibited the growth of seven important phytopathogens. The inhibition rate ranged from 75.51 to 100% respectively. Solid phase micro-extraction coupled with gas chromatography tandem mass spectrometry proved that the antifungal volatile was dimethyl disulfide. Dimethyl disulfide can inhibit the germination of Aspergillus flavus , and severely destroy the cell structures under scanning electron microscopy. Conclusions S. marcescens Pt-3 with multiple functions will provide novel agent for the production of bioactive fertilizer with P-solubilizing and fungal pathogens control activity.
    Type of Medium: Online Resource
    ISSN: 1471-2180
    URL: Article
    DOI: 10.1186/s12866-021-02434-5
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2022
    detail.hit.zdb_id: 2041505-9
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  • 8
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    The differential expression patterns of paralogs in response to stresses indicate expression and sequence divergences
    Springer Science and Business Media LLC ; 2020
    In:  BMC Plant Biology Vol. 20, No. 1 ( 2020-12)
    Details
    In: BMC Plant Biology, Springer Science and Business Media LLC, Vol. 20, No. 1 ( 2020-12)
    Abstract: Theoretically, paralogous genes generated through whole genome duplications should share identical expression levels due to their identical sequences and chromatin environments. However, functional divergences and expression differences have arisen due to selective pressures throughout evolution. A comprehensive investigation of the expression patterns of paralogous gene pairs in response to various stresses and a study of correlations between the expression levels and sequence divergences of the paralogs are needed. Results In this study, we analyzed the expression patterns of paralogous genes under different types of stress and investigated the correlations between the expression levels and sequence divergences of the paralogs. We analyzed the differential expression patterns of the paralogs under four different types of stress (drought, cold, infection, and herbivory) and classified them into three main types according to their expression patterns. We then further analyzed the differential expression patterns under various degrees of stress and constructed corresponding co-expression networks of differentially expressed paralogs and transcription factors. Finally, we investigated the correlations between the expression levels and sequence divergences of the paralogs and identified positive correlations between expression level and sequence divergence. With regard to sequence divergence, we identified correlations between selective pressures and phylogenetic relationships. Conclusions These results shed light on differential expression patterns of paralogs in response to environmental stresses and are helpful for understanding the relationships between expression levels and sequences divergences.
    Type of Medium: Online Resource
    ISSN: 1471-2229
    URL: Article
    DOI: 10.1186/s12870-020-02460-x
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2020
    detail.hit.zdb_id: 2059868-3
    SSG: 12
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  • 9
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    Online Resource
    Table_1.xlsx
    Frontiers Media SA ; 2023
    In:  Frontiers in Plant Science Vol. 14 ( 2023-2-20)
    Details
    In: Frontiers in Plant Science, Frontiers Media SA, Vol. 14 ( 2023-2-20)
    Abstract: As one of the world’s top three popular non-alcoholic beverages, tea is economically and culturally valuable. Xinyang Maojian, this elegant green tea, is one of the top ten famous tea in China and has gained prominence for thousands of years. However, the cultivation history of Xinyang Maojian tea population and selection signals of differentiation from the other major variety Camellia sinensis var. assamica ( CSA ) remain unclear. We newly generated 94 Camellia sinensis ( C . sinensis ) transcriptomes including 59 samples in the Xinyang area and 35 samples collected from 13 other major tea planting provinces in China. Comparing the very low resolution of phylogeny inferred from 1785 low-copy nuclear genes with 94  C. sinensis samples, we successfully resolved the phylogeny of C. sinensis samples by 99,115 high-quality SNPs from the coding region. The sources of tea planted in the Xinyang area were extensive and complex. Specifically, Shihe District and Gushi County were the two earliest tea planting areas in Xinyang, reflecting a long history of tea planting. Furthermore, we identified numerous selection sweeps during the differentiation of CSA and CSS and these positive selection genes are involved in many aspects such as regulation of secondary metabolites synthesis, amino acid metabolism, photosynthesis, etc. Numerous specific selective sweeps of modern cultivars were annotated with functions in various different aspects, indicating the CSS and CSA populations possibly underwent independent specific domestication processes. Our study indicated that transcriptome-based SNP-calling is an efficient and cost-effective method in untangling intraspecific phylogenetic relationships. This study provides a significant understanding of the cultivation history of the famous Chinese tea Xinyang Maojian and unravels the genetic basis of physiological and ecological differences between the two major tea subspecies.
    Type of Medium: Online Resource
    ISSN: 1664-462X
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    URL: Article
    DOI: 10.3389/fpls.2023.1114284
    DOI: 10.3389/fpls.2023.1114284.s001
    DOI: 10.3389/fpls.2023.1114284.s002
    DOI: 10.3389/fpls.2023.1114284.s003
    DOI: 10.3389/fpls.2023.1114284.s004
    DOI: 10.3389/fpls.2023.1114284.s005
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2687947-5
    detail.hit.zdb_id: 2613694-6
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  • 10
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    Numerical simulation of solid particle erosion in the gas-liquid flow of key pipe fittings in shale gas fields
    Elsevier BV ; 2023
    In:  Case Studies in Thermal Engineering Vol. 42 ( 2023-02), p. 102742-
    Details
    In: Case Studies in Thermal Engineering, Elsevier BV, Vol. 42 ( 2023-02), p. 102742-
    Type of Medium: Online Resource
    ISSN: 2214-157X
    URL: Article
    DOI: 10.1016/j.csite.2023.102742
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2732684-6
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