In:
Liver International, Wiley, Vol. 34, No. 3 ( 2014-03), p. 367-378
Abstract:
There is a demand for more sensitive, specific and predictive biomarkers for drug‐induced liver injury ( DILI ) than the gold standard used today, alanine aminotransferase ( ALT ). The aim of this study was to qualify novel DILI biomarkers (keratin‐18 markers M65/M30, micro RNA ‐122, glutamate dehydrogenase and alpha‐foetoprotein) in human DILI . Methods Levels of the novel biomarkers were measured by enzyme‐linked immunosorbent assay or real‐time quantitative reverse‐transcription PCR ( qRT ‐PCR) in two human DILI cohorts: a human volunteer study with acetaminophen and a human immunodeficiency virus (HIV)/tuberculosis (TB) study. Results In the acetaminophen study, serum M65 and micro RNA ‐122 levels were significantly increased at an earlier time point than ALT . Furthermore, the maximal elevation of M65 and micro RNA ‐122 exceeded the increase in ALT . In the HIV / TB study, all the analysed novel biomarkers increased after 1 week of treatment. In contrast to ALT , the novel biomarkers remained stable in a human cohort with exercise‐induced muscular injury. Conclusions M65 and micro RNA ‐122 are potential biomarkers of DILI superior to ALT with respect to sensitivity and specificity.
Type of Medium:
Online Resource
ISSN:
1478-3223
,
1478-3231
DOI:
10.1111/liv.2014.34.issue-3
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2124684-1
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