In:
International Archives of Allergy and Immunology, S. Karger AG, Vol. 146, No. 1 ( 2008), p. 19-26
Abstract:
〈 i 〉 Background: 〈 /i 〉 Fruits are a major cause of food allergy in adults. Lipid transfer proteins (LTP) are implicated in severe allergic reactions to fruits, but little is known about LTP content in different cultivars. 〈 i 〉 Objective: 〈 /i 〉 Determination of the levels of LTP in a wide range of apple cultivars. 〈 i 〉 Methods: 〈 /i 〉 LTP was measured in apples from 53 cultivars grown in Italy and 35 grown in The Netherlands, using three different immunoassays: a competitive ELISA (cELISA), a sandwich ELISA (sELISA) and a RAST inhibition (RI). Selected cultivars were evaluated using the basophil histamine release test (BHR), skin prick test (SPT) and double-blind, placebo-controlled food challenge (DBPCFC). 〈 i 〉 Results: 〈 /i 〉 LTP levels measured with the three immunoassays were significantly correlated, as judged by Pearson’s correlation (0.61 〈 Rp 〈 0.65; p 〈 0.0001), but differed with respect to the actual quantities: 3.4–253.2 (sELISA), 2.7–120.2 (cELISA) and 0.4–47.3 µg/g tissue (RI). Between cultivars, LTP titers varied over about a two-log range. Pilot in vitro and in vivo biological testing (BHR, SPT and DBPCFC) with selected cultivars supported the observed differences in LTP levels. 〈 i 〉 Conclusions: 〈 /i 〉 Around 100-fold differences in LTP levels exist between apple cultivars. Whether the lowest observed levels of LTP warrant designation as hypo-allergenic requires more extensive confirmation by oral challenges. Determination of cultivar variation in LTP levels provides important information for growers and consumers. Comparison to earlier reported Mal d 1 levels in the same cultivars reveals that a designation as low allergenic does not always coincide for both allergens.
Type of Medium:
Online Resource
ISSN:
1018-2438
,
1423-0097
Language:
English
Publisher:
S. Karger AG
Publication Date:
2008
detail.hit.zdb_id:
1482722-0
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