In:
Cancer Research, American Association for Cancer Research (AACR), Vol. 82, No. 12_Supplement ( 2022-06-15), p. 3871-3871
Abstract:
While classic, chromogenic immunohistochemistry (IHC) remains a mainstay in clinical diagnostics, it can be limiting in the era of immuno-oncology. In the field of immuno-oncology, where access to tissue samples may be limited, there is a need for an accurate interpretation of the sociology and in-depth phenotyping of the cells in the tumor microenvironment that requires a higher level of targets to investigate. Cytotoxic T cells are the main effector cells against tumors. Conversely, T-regulatory cells play a more pro-tumor role by suppressing cytotoxic T cell activation. The importance of the balance and tumor infiltration of these two cell populations has repeatedly been demonstrated in the literature. Due to this, Cerba Research was driven to develop an immunofluorescent multiplex to allow cytotoxic and regulatory T cells detection on the same tissue section for use in solid tumor clinical trials. Following our standard workflow, we developed a CD3-CD8-FoxP3 IHC multiplex (Histoprofile®- T-reg light). This panel allows for analysis of cytotoxic T-cells (CD3+/CD8+/FoxP3-), T helper cells (CD3+/CD8-/FoxP3-) and T regulatory cells (CD3+/CD8-/FoxP3+). Then following our standard process, we performed an analytical validation on a range of formalin fixed paraffin embedded solid tumors (including but not limited to Non Small Cell Lung Cancer, Head and Neck Squamous Cell Carcinoma, Colorectal Cancer and Breast Cancer). Specificity was verified by a pathologist on control tonsil tissue. The robustness of the protocol on a range of solid tumors was verified by the pathologist on tissue blocks and tumor microarrays. Precision tests (intra- and inter-assay) demonstrated a high level of panel reproducibility in solid tumor tissues - CD3 (CV 21.24%), CD8 (CV 19.5%), FoxP3 (CV 13.13%). Using Halo® software (Indica Labs), spatial analysis of the populations of interest can be appreciated in the tumor and stroma compartments. Cerba Research’s Histoprofile®- T-reg light panel passed all the analytical validation criteria for use in clinical studies. Cerba Research can provide this off the shelf panel in solid tumor clinical studies, allowing an elevated analysis of multiple immune biomarkers on a single tissue slide. Citation Format: Marie Gérus-Durand, Elena Baranova, Maroua Tliba, Sabine Iglesias, Manon Motte, Renaud Burrer, Amanda Finan-Marchi. Solid tumor analytical validation of a T-regulatory immunohistochemistry multiplex for clinical studies [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2022; 2022 Apr 8-13. Philadelphia (PA): AACR; Cancer Res 2022;82(12_Suppl):Abstract nr 3871.
Type of Medium:
Online Resource
ISSN:
1538-7445
DOI:
10.1158/1538-7445.AM2022-3871
Language:
English
Publisher:
American Association for Cancer Research (AACR)
Publication Date:
2022
detail.hit.zdb_id:
2036785-5
detail.hit.zdb_id:
1432-1
detail.hit.zdb_id:
410466-3
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