In:
Development, Growth & Differentiation, Wiley, Vol. 56, No. 9 ( 2014-12), p. 595-604
Abstract:
In several laboratory animals and humans, adipose tissue‐derived mesenchymal stem cells ( ASC ) are of considerable interest because they are easy to harvest and can generate a huge proliferation of cells from a small quantity of fat. In this study, we investigated: (i) the expression patterns of reprogramming‐related genes in porcine ASC ; and (ii) whether ASC can be a suitable donor cell type for generating cloned pigs. For these experiments, ASC , adult skin fibroblasts ( AF ) and fetal fibroblasts ( FF ) were derived from a 4‐year‐old female miniature pig. The ASC expressed cell‐surface markers characteristic of stem cells, and underwent in vitro differentiation when exposed to specific differentiation‐inducing conditions. Expression of DNA methyltransferase ( DNMT )1 in ASC was similar to that in AF , but the highest expression of the DNMT3B gene was observed in ASC . The expression of OCT4 was significantly higher in FF and ASC than in AF ( P 〈 0.05), and SOX2 showed significantly higher expression in ASC than in the other two cell types ( P 〈 0.05). After somatic cell nuclear transfer ( SCNT ), the development rate of cloned embryos derived from ASC was comparable to the development of those derived using FF . Total cell numbers of blastocysts derived using ASC and FF were significantly higher than in embryos made with AF . The results demonstrated that ASC used for SCNT have a potential comparable to those of AF and FF in terms of embryo in vitro development and blastocyst formation.
Type of Medium:
Online Resource
ISSN:
0012-1592
,
1440-169X
DOI:
10.1111/dgd.2014.56.issue-9
Language:
English
Publisher:
Wiley
Publication Date:
2014
detail.hit.zdb_id:
2020067-5
SSG:
12
Permalink