In:
Frontiers in Plant Science, Frontiers Media SA, Vol. 13 ( 2022-5-31)
Abstract:
Cunninghamia lanceolata is an essential timber species that provide 20%–30% raw materials for China’s timber industry. Although a few transcriptomes have been published in C. lanceolata , full-length mRNA transcripts and regulatory mechanisms behind the cellulose and lignin biosynthesis have not been thoroughly investigated. Here, PacBio Iso-seq and RNA-seq analyses were adapted to identify the full-length and differentially expressed transcripts along a developmental gradient from apex to base of C. lanceolata shoots. A total of 48,846 high-quality full-length transcripts were obtained, of which 88.0% are completed transcriptome based on benchmarking universal single-copy orthologs (BUSCO) assessment. Along stem developmental gradient, 18,714 differentially expressed genes (DEGs) were detected. Further, 28 and 125 DEGs were identified as enzyme-coding genes of cellulose and lignin biosynthesis, respectively. Moreover, 57 transcription factors (TFs), including MYB and NAC , were identified to be involved in the regulatory network of cellulose and lignin biosynthesis through weighted gene co-expression network analysis (WGCNA). These TFs are composed of a comparable regulatory network of secondary cell wall formation in angiosperms, revealing a similar mechanism may exist in gymnosperms. Further, through qRT-PCR, we also investigated eight specific TFs involved in compression wood formation. Our findings provide a comprehensive and valuable source for molecular genetics breeding of C. lanceolata and will be beneficial for molecular-assisted selection.
Type of Medium:
Online Resource
ISSN:
1664-462X
DOI:
10.3389/fpls.2022.883720
DOI:
10.3389/fpls.2022.883720.s001
DOI:
10.3389/fpls.2022.883720.s002
DOI:
10.3389/fpls.2022.883720.s003
DOI:
10.3389/fpls.2022.883720.s004
DOI:
10.3389/fpls.2022.883720.s005
DOI:
10.3389/fpls.2022.883720.s006
DOI:
10.3389/fpls.2022.883720.s007
DOI:
10.3389/fpls.2022.883720.s008
DOI:
10.3389/fpls.2022.883720.s009
DOI:
10.3389/fpls.2022.883720.s010
DOI:
10.3389/fpls.2022.883720.s011
Language:
Unknown
Publisher:
Frontiers Media SA
Publication Date:
2022
detail.hit.zdb_id:
2687947-5
detail.hit.zdb_id:
2613694-6
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