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  • 1
    In: Veterinary Sciences, MDPI AG, Vol. 10, No. 5 ( 2023-05-11), p. 341-
    Abstract: Tengchong Snow chickens are one of the most precious, black-boned chickens in Yunnan province and usually produce black meat. However, we found a small number of white meat traits in the chicken population during feeding. In order to determine the pattern of melanin deposition and the molecular mechanism of formation in the Tengchong Snow chicken, we measured the luminance value (L value) and melanin content in the skin of black meat chickens (Bc) and white meat chickens (Wc) using a color colorimeter, ELISA kit, and enzyme marker. The results showed that the L value of skin tissues in black meat chickens was significantly lower than that of white meat chickens, and the L value of skin tissues gradually increased with an increase in age. The melanin content of skin tissues in black meat chickens was higher than that of white meat chickens, and melanin content in the skin tissues gradually decreased with an increase in age, but this difference was not significant (p 〉 0.05); the L value of skin tissues in black meat chickens was negatively correlated with melanin content, and the correlation coefficient was mostly above −0.6. In addition, based on the phenotypic results, we chose to perform the comparative transcriptome profiling of skin tissues at 90 days of age. We screened a total of 44 differential genes, of which 32 were upregulated and 12 were downregulated. These DEGs were mainly involved in melanogenesis, tyrosine metabolism and RNA transport. We identified TYR, DCT, and EDNRB2 as possible master effector genes for skin pigmentation in Tengchong Snow black meat chickens through DEGs analysis. Finally, we measured the mRNA of TYR, DCT, MC1R, EDNRB2, GPR143, MITF, and TYRP1 genes through a quantitative real-time polymerase chain reaction (qPCR) and found that the mRNA of all the above seven genes decreased with increasing age. In conclusion, our study initially constructed an evaluation system for the black-boned traits of Tengchong Snow chickens and found key candidate genes regulating melanin deposition, which could provide an important theoretical basis for the selection and breeding of black-boned chickens.
    Type of Medium: Online Resource
    ISSN: 2306-7381
    Language: English
    Publisher: MDPI AG
    Publication Date: 2023
    detail.hit.zdb_id: 2768971-2
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  • 2
    Online Resource
    Online Resource
    Walter de Gruyter GmbH ; 2023
    In:  Translational Neuroscience Vol. 14, No. 1 ( 2023-06-07)
    In: Translational Neuroscience, Walter de Gruyter GmbH, Vol. 14, No. 1 ( 2023-06-07)
    Abstract: Accumulating evidence has suggested that thyroid hormone levels affect the prognosis of acute ischemic stroke (AIS), but the results have been inconsistent. Methods Basic data, neural scale scores, thyroid hormone levels, and other laboratory examination data of AIS patients were collected. The patients were divided into excellent and poor prognosis group at discharge and 90 days after discharge. Logistic regression models were applied to evaluate the relationship between thyroid hormone levels and prognosis. A subgroup analysis was performed based on stroke severity. Results A number of 441 AIS patients were included in this study. Those in the poor prognosis group were older, with higher blood sugar levels, higher free thyroxine (FT4) levels, and severe stroke (all p 〈 0.05) at baseline. Free thyroxine (FT4) showed a predictive value (all p 〈 0.05) for prognosis in the model adjusted for age, gender, systolic pressure, and glucose level. However, after adjustment for types and severity of stroke, FT4 showed insignificant associations. In the severe subgroup at discharge, the change in FT4 was statistically significant ( p = 0.015), odds ratio (95% confidence interval) = 1.394 (1.068–1.820) but not in the other subgroups. Conclusions High-normal FT4 serum levels in patients with severe stroke receiving conservative medical treatment at admission may indicate a worse short-term prognosis.
    Type of Medium: Online Resource
    ISSN: 2081-6936
    Language: English
    Publisher: Walter de Gruyter GmbH
    Publication Date: 2023
    detail.hit.zdb_id: 2581219-1
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  • 3
    In: Frontiers in Psychiatry, Frontiers Media SA, Vol. 11 ( 2020-11-23)
    Abstract: This study aimed to explore the psychological situation and the influence of the outbreak of COVID-19 on college students. An online questionnaire survey was conducted among 3,092 Chinese college students who were quarantined at home as a result of the COVID-19 pandemic. The survey tools included the Generalized Anxiety Disorder 7-Item Scale (GAD-7), the Perceived Stress Scale (PSS-10), and the Self-Rating Scale of Sleep (SRSS). Of all the respondents, the prevalence of anxiety symptoms, sleep problems, any of the two, and both of the two, were 16.8, 13.5, 25.1, and 5.3%, respectively. Of the participants, 43.7% of the college students had higher perceived stress. Factors associated with anxiety symptoms included reading the daily news with higher frequency (1–3 times; 4–7 times; more than 7 times), having sleep problems, higher stress, and carelessness with the number of remaining masks. Factors associated with sleep problems included postgraduates, reading the news with higher frequency daily (1–3 times), the frequency of going out per week (1–3 times), having anxiety symptoms and higher stress. Factors associated with higher perceived stress included reading the daily news with higher frequency (4–7 times), anxiety about the number of remaining masks (1–10; more than 20), having anxiety symptoms, and having sleep problems. The prevalence of anxiety symptoms, sleep problems, and higher perceived stress among college students was high during the COVID-19 outbreak. Particular attention should be paid to psychological support for college students quarantined at home, especially those at high risk of psychological problems.
    Type of Medium: Online Resource
    ISSN: 1664-0640
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2020
    detail.hit.zdb_id: 2564218-2
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  • 4
    Online Resource
    Online Resource
    Indian Pharmaceutical Association - IPA ; 2020
    In:  Indian Journal of Pharmaceutical Sciences Vol. 82 ( 2020)
    In: Indian Journal of Pharmaceutical Sciences, Indian Pharmaceutical Association - IPA, Vol. 82 ( 2020)
    Type of Medium: Online Resource
    Language: Unknown
    Publisher: Indian Pharmaceutical Association - IPA
    Publication Date: 2020
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  • 5
    In: Journal of Cleaner Production, Elsevier BV, Vol. 313 ( 2021-09), p. 127896-
    Type of Medium: Online Resource
    ISSN: 0959-6526
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 1179393-4
    detail.hit.zdb_id: 2029338-0
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  • 6
    In: Food and Bioproducts Processing, Elsevier BV, Vol. 142 ( 2023-11), p. 40-49
    Type of Medium: Online Resource
    ISSN: 0960-3085
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2023
    detail.hit.zdb_id: 2008001-3
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  • 7
    Online Resource
    Online Resource
    Frontiers Media SA ; 2023
    In:  Frontiers in Neurology Vol. 14 ( 2023-6-15)
    In: Frontiers in Neurology, Frontiers Media SA, Vol. 14 ( 2023-6-15)
    Abstract: Many patients with acute ischemic stroke (AIS) cannot undergo thrombolysis or thrombectomy because they have missed the time window or do not meet the treatment criteria. In addition, there is a lack of an available tool to predict the prognosis of patients with standardized treatment. This study aimed to develop a dynamic nomogram to predict the 3-month poor outcomes in patients with AIS. Methods This was a retrospective multicenter study. We collected the clinical data of patients with AIS who underwent standardized treatment at the First People's Hospital of Lianyungang from 1 October 2019 to 31 December 2021 and at the Second People's Hospital of Lianyungang from 1 January 2022 to 17 July 2022. Baseline demographic, clinical, and laboratory information of patients were recorded. The outcome was the 3-month modified Rankin Scale (mRS) score. The least absolute shrinkage and selection operator regression were used to select the optimal predictive factors. Multiple logistic regression was performed to establish the nomogram. A decision curve analysis (DCA) was applied to assess the clinical benefit of the nomogram. The calibration and discrimination properties of the nomogram were validated by calibration plots and the concordance index. Results A total of 823 eligible patients were enrolled. The final model included gender (male; OR 0.555; 95% CI, 0.378–0.813), systolic blood pressure (SBP; OR 1.006; 95% CI, 0.996–1.016), free triiodothyronine (FT3; OR 0.841; 95% CI, 0.629–1.124), National Institutes of Health stroke scale (NIHSS; OR 18.074; 95% CI, 12.264–27.054), Trial of Org 10172 in Acute Stroke Treatment (TOAST; cardioembolic (OR 0.736; 95% CI, 0.396–1.36); and other subtypes (OR 0.398; 95% CI, 0.257–0.609). The nomogram showed good calibration and discrimination (C-index, 0.858; 95% CI, 0.830–0.886). DCA confirmed the clinical usefulness of the model. The dynamic nomogram can be obtained at the website: predict model (90-day prognosis of AIS patients) . Conclusion We established a dynamic nomogram based on gender, SBP, FT3, NIHSS, and TOAST, which calculated the probability of 90-day poor prognosis in AIS patients with standardized treatment.
    Type of Medium: Online Resource
    ISSN: 1664-2295
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2564214-5
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  • 8
    In: Frontiers in Aging Neuroscience, Frontiers Media SA, Vol. 15 ( 2023-5-24)
    Abstract: The prevalence of cerebral small vessel disease (CSVD) is increasing due to the accelerating global aging process, resulting in a substantial burden on all countries, as cognitive dysfunction associated with CSVD is also on the rise. Clock genes have a significant impact on cognitive decline and dementia. Furthermore, the pattern of DNA methylation in clock genes is strongly associated with cognitive impairment. Thus, the aim of this study was to explore the connection between DNA promoter methylation of PER1 and CRY1 and cognitive dysfunction in patients with CSVD. Methods We recruited patients with CSVD admitted to the Geriatrics Department of the Lianyungang Second People’s Hospital between March 2021 and June 2022. Based on their Mini-Mental State Examination score, patients were categorized into two groups: 65 cases with cognitive dysfunction and 36 cases with normal cognitive function. Clinical data, 24-h ambulatory blood pressure monitoring parameters, and CSVD total load scores were collected. Moreover, we employed methylation-specific PCR to analyze the peripheral blood promoter methylation levels of clock genes PER1 and CRY1 in all CSVD patients who were enrolled. Finally, we used binary logistic regression models to assess the association between the promoter methylation of clock genes (PER1 and CRY1) and cognitive dysfunction in patients with CSVD. Results (1) A total of 101 individuals with CSVD were included in this study. There were no statistical differences between the two groups in baseline clinical data except MMSE and AD8 scores. (2) After B/H correction, the promoter methylation rate of PER1 was higher in the cognitive dysfunction group than that in the normal group, and the difference was statistically significant (adjusted p & lt; 0.001). (3) There was no significant correlation between the promoter methylation rates of PER1 and CRY1 in peripheral blood and circadian rhythm of blood pressure ( p   & gt; 0.05). (4) Binary logistic regression models showed that the influence of promoter methylation of PER1 and CRY1 on cognitive dysfunction were statistically significant in Model 1 ( p & lt; 0.001; p = 0.025), and it still existed after adjusting for confounding factors in Model 2. Patients with the promoter methylation of PER1 gene ( OR = 16.565, 95% CI , 4.057–67.628; p & lt; 0.001) and the promoter methylation of CRY1 gene ( OR = 6.017, 95% CI , 1.290–28.069; p = 0.022) were at greater risk of cognitive dysfunction compared with those with unmethylated promoters of corresponding genes in Model 2. Conclusion The promoter methylation rate of PER1 gene was higher in the cognitive dysfunction group among CSVD patients. And the hypermethylation of the promoters of clock genes PER1 and CRY1 may be involved in affecting cognitive dysfunction in patients with CSVD.
    Type of Medium: Online Resource
    ISSN: 1663-4365
    Language: Unknown
    Publisher: Frontiers Media SA
    Publication Date: 2023
    detail.hit.zdb_id: 2558898-9
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  • 9
    In: Atmospheric Research, Elsevier BV, Vol. 255 ( 2021-06), p. 105558-
    Type of Medium: Online Resource
    ISSN: 0169-8095
    Language: English
    Publisher: Elsevier BV
    Publication Date: 2021
    detail.hit.zdb_id: 2012396-6
    detail.hit.zdb_id: 233023-4
    SSG: 16,13
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  • 10
    Online Resource
    Online Resource
    Informa UK Limited ; 2022
    In:  Neuropsychiatric Disease and Treatment Vol. Volume 18 ( 2022-03), p. 487-498
    In: Neuropsychiatric Disease and Treatment, Informa UK Limited, Vol. Volume 18 ( 2022-03), p. 487-498
    Type of Medium: Online Resource
    ISSN: 1178-2021
    Language: English
    Publisher: Informa UK Limited
    Publication Date: 2022
    detail.hit.zdb_id: 2180554-4
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