In:
Angewandte Chemie, Wiley, Vol. 130, No. 20 ( 2018-05-14), p. 5722-5727
Abstract:
Live‐cell labeling, super‐resolution microscopy, single‐molecule applications, protein localization, or chemically induced assembly are emerging approaches, which require specific and very small interaction pairs. The minimal disturbance of protein function is essential to derive unbiased insights into cellular processes. Herein, we define a new class of hexavalent N ‐nitrilotriacetic acid ( hexa NTA) chelators, displaying the highest affinity and stability of all NTA‐based small interaction pairs described so far. Coupled to bright organic fluorophores with fine‐tuned photophysical properties, the super‐chelator probes were delivered into human cells by chemically gated nanopores. These super‐chelators permit kinetic profiling, multiplexed labeling of His 6 ‐ and His 12 ‐tagged proteins as well as single‐molecule‐based super‐resolution imaging.
Type of Medium:
Online Resource
ISSN:
0044-8249
,
1521-3757
DOI:
10.1002/ange.v130.20
DOI:
10.1002/ange.201800827
Language:
English
Publisher:
Wiley
Publication Date:
2018
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505868-5
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506609-8
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514305-6
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505872-7
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1479266-7
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505867-3
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506259-7
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