In:
Scientific Reports, Springer Science and Business Media LLC, Vol. 10, No. 1 ( 2020-09-21)
Abstract:
The large catalytic subunit of acetohydroxyacid synthase (AHAS, EC 2.2.1.6) of Thermotoga maritima (TmcAHAS) was prepared in this study. It possesses high specific activity and excellent stability. The protein and a whole cell catalyst overexpressing the protein were applied to the preparation of α-hydroxyketones including acetoin (AC), 3-hydroxy-2-pentanone (HP), and ( R )-phenylacetylcarbinol ( R- PAC). The results show that AC and HP could be produced in high yields (84% and 62%, respectively), while R -PAC could be synthesized in a high yield (about 78%) with an R / S ratio of 9:1. Therefore, TmcAHAS and the whole cell catalyst overexpressing the protein could be practically useful bio-catalysts in the preparation of α-hydroxyketones including AC, HP, and R -PAC. To the best of our knowledge, this is the first time that bacterial AHAS was used as a catalyst to prepare HP with a good yield, and also the first time that TmcAHAS was employed to synthesize AC and R -PAC.
Type of Medium:
Online Resource
ISSN:
2045-2322
DOI:
10.1038/s41598-020-72416-6
Language:
English
Publisher:
Springer Science and Business Media LLC
Publication Date:
2020
detail.hit.zdb_id:
2615211-3
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