In:
PLOS ONE, Public Library of Science (PLoS), Vol. 16, No. 7 ( 2021-7-19), p. e0254632-
Abstract:
Superoxide radicals and other reactive oxygen species (ROS) are implicated in influenza A virus-induced inflammation. In this in vitro study, we evaluated the effects of TG6-44, a novel quinazolin-derived myeloperoxidase-specific ROS inhibitor, on influenza A virus (A/X31) infection using THP-1 lung monocytic cells and freshly isolated peripheral blood mononuclear cells (PBMC). TG6-44 significantly decreased A/X31-induced ROS and virus-induced inflammatory mediators in THP-1 cells (IL-6, IFN-γ, MCP-1, TNF-α, MIP-1β) and in human PBMC (IL-6, IL-8, TNF-α, MCP-1). Interestingly, TG6-44-treated THP-1 cells showed a decrease in percent cells expressing viral nucleoprotein, as well as a delay in translocation of viral nucleoprotein into the nucleus. Furthermore, in influenza A virus-infected cells, TG6-44 treatment led to suppression of virus-induced cell death as evidenced by decreased caspase-3 activation, decreased proportion of Annexin V + PI + cells, and increased Bcl-2 phosphorylation. Taken together, our results demonstrate the anti-inflammatory and anti-infective effects of TG6-44.
Type of Medium:
Online Resource
ISSN:
1932-6203
DOI:
10.1371/journal.pone.0254632
DOI:
10.1371/journal.pone.0254632.g001
DOI:
10.1371/journal.pone.0254632.g002
DOI:
10.1371/journal.pone.0254632.g003
DOI:
10.1371/journal.pone.0254632.g004
DOI:
10.1371/journal.pone.0254632.g005
DOI:
10.1371/journal.pone.0254632.g006
DOI:
10.1371/journal.pone.0254632.s001
DOI:
10.1371/journal.pone.0254632.s002
DOI:
10.1371/journal.pone.0254632.s003
DOI:
10.1371/journal.pone.0254632.s004
DOI:
10.1371/journal.pone.0254632.s005
Language:
English
Publisher:
Public Library of Science (PLoS)
Publication Date:
2021
detail.hit.zdb_id:
2267670-3
Permalink