In:
Journal of Biomedical Materials Research Part B: Applied Biomaterials, Wiley, Vol. 110, No. 11 ( 2022-11), p. 2387-2398
Abstract:
There are no small‐diameter, long artificial vascular grafts for below‐knee bypass surgery in chronic limb‐threatening ischemia. We have developed tissue‐engineered vascular grafts called “Biotubes®” using a completely autologous approach called in‐body tissue architecture (iBTA). This study aimed at pre‐implantation evaluation of Biotube and its in vivo preparation device, Biotube Maker, for use in below‐knee bypass surgery. Forty nine makers were subcutaneously embedded into 17 goats for predetermined periods (1, 2, or 3 months). All makers produced Biotubes as designed without inflammation over all periods, with the exception of a few cases with minor defects (success rate: 94%). Small hole formation occurred in only a few cases. All Biotubes obtained had an inner diameter of 4 mm and a length of 51 to 52 cm with a wall thickness of 594 ± 97 μm. All Biotubes did not kink when completely bent under an internal pressure of 100 mmHg and did not leak without any deformation under a water pressure of 200 mmHg. Their burst strength was 2409 ± 473 mmHg, and suture retention strength was 1.75 ± 0.27 N, regardless of the embedding period, whereas tensile strength increased from 7.5 ± 1.3 N at 1 month to 9.7 ± 2.0 N at 3 months with the embedding period. The amount of water leakage from the needle holes prepared in the Biotube wall was approximately 1/7th of that in expanded polytetrafluoroethylene vascular grafts. The Biotubes could be easily connected to each other without cutting or anastomosis leaks. They could be stored for at least 1 year at room temperature. This study confirmed that even Biotubes formed 1 month after embedding of Biotube Makers had properties comparable to arteries.
Type of Medium:
Online Resource
ISSN:
1552-4973
,
1552-4981
DOI:
10.1002/jbm.b.v110.11
Language:
English
Publisher:
Wiley
Publication Date:
2022
detail.hit.zdb_id:
2130917-6
SSG:
12
Permalink