In:
Journal of Bacteriology, American Society for Microbiology, Vol. 177, No. 17 ( 1995-09), p. 5169-5175
Kurzfassung:
The 6.2-kbp DNA fragment encoding the enzymes in the porphyrin synthesis pathway of a cellulolytic anaerobe, Clostridium josui, was cloned into Escherichia coli and sequenced. This fragment contained four hem genes, hemA, hemC, hemD, and hemB, in order, which were homologous to the corresponding genes from E. coli and Bacillus subtilis. A typical promoter sequence was found only upstream of hemA, suggesting that these four genes were under the control of this promoter as an operon. The hemA and hemD genes cloned from C. josui were able to complement the hemA and hemD mutations, respectively, of E. coli. The COOH-terminal region of C. josui HemA and the NH2-terminal region of C. josui HemD were homologous to E. coli CysG (Met-1 to Leu-151) and to E. coli CysG (Asp-213 to Phe-454) and Pseudomonas denitrificans CobA, respectively. Furthermore, the cloned 6.2-kbp DNA fragment complemented E. coli cysG mutants. These results suggested that both C. josui hemA and hemD encode bifunctional enzymes.
Materialart:
Online-Ressource
ISSN:
0021-9193
,
1098-5530
DOI:
10.1128/jb.177.17.5169-5175.1995
Sprache:
Englisch
Verlag:
American Society for Microbiology
Publikationsdatum:
1995
ZDB Id:
1481988-0
SSG:
12
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