In:
FEBS Open Bio, Wiley, Vol. 7, No. 11 ( 2017-11), p. 1715-1721
Abstract:
Inovirus XacF1 (7325 nucleotides) is integrated into the genome of Xanthomonas citri pv. citri ( Xcc ) strains at the host dif site ( att B) by the host XerC/D recombination system. The XacF1 att P sequence is located within the coding region of ORF 12, a possible phage regulator. After integration, this open reading frame ( ORF ) is split into two pieces on the host genome. We examined dynamic integration/excision of XacF1 in Xcc strain MAFF 301080 and found that the integration started at 4 h postinfection (p.i.) and peaked at 12 h p.i. Thereafter, the ratio of integrated to free forms remained constant, suggesting equilibrium of integration and excision of XacF1 in the host genome. However, the integrated state became very unstable following a 5′‐deletion of ORF 12 in XacF1, suggesting that ORF 12 plays a key role in the integration cycle of XacF1 in Xcc strains.
Type of Medium:
Online Resource
ISSN:
2211-5463
,
2211-5463
DOI:
10.1002/feb4.2017.7.issue-11
DOI:
10.1002/2211-5463.12312
Language:
English
Publisher:
Wiley
Publication Date:
2017
detail.hit.zdb_id:
2651702-4
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