In:
Small, Wiley, Vol. 19, No. 26 ( 2023-06)
Abstract:
This work reports a portable, origami‐type paper device with a plasmonic fluor‐labeled microneedle sensing module for the multiplexed quantification of anemia biomarkers in whole blood. Sequential steps, including serum separation, target enrichment, and multiplexed readout by a gel imager, are rapidly accomplished with the flexible and highly integrated device. The microneedle array enabled efficient sampling of trace targets from ng mL −1 to pg mL −1 level. Combined with the plasmonic fluor label, the signal is improved by ≈7.6 folds compared with the flat substrate‐based assay. The device is applied to simultaneously quantify hemoglobin (Hb), ferritin, folic acid (FA), and vitamin B 12 (VB 12 ), which are four anemia biomarkers distributed in different environments with different concentration ranges. Featured by the small sample volume (150 µL), short assay time (20 min), low cost (2 $), robust stability, and user‐friendliness, the device is promising for the rapid and accurate diagnosis of anemia in real practice.
Type of Medium:
Online Resource
ISSN:
1613-6810
,
1613-6829
DOI:
10.1002/smll.202300464
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2168935-0
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