In:
Prenatal Diagnosis, Wiley, Vol. 33, No. 5 ( 2013-05), p. 502-508
Abstract:
What's already known about this topic? The gold standard analysis of CVS includes the cytogenetic analysis of cytotrophoblast (STC) and mesenchyme (LTC). QF‐PCR is a widely used method for rapid aneuploidy detection in prenatal samples. The application of QF‐PCR on CVS is problematic because discrepant results with karyotype have been reported. A combined approach QF‐PCR +; LTC was proposed instead of STC +; LTC to minimize the risk of discrepant results and achieve a simplification of cytogenetic methods and procedures for the analysis of CVS. What does this study add? Compared with the STC + LTC combined method, QF‐PCR + LTC is associated with a cumulative risk of false‐negative result ranging from ~1/3100 to 1/4400 (depending on the sex chromosome investigation), similar cost/sample (considering a high throughput cytogenetic lab organization and a CE‐IVD marked QF‐PCR assay) and identical reporting time because preliminary result communications based on only QF‐PCR test are generally discouraged as routine practice.
Type of Medium:
Online Resource
ISSN:
0197-3851
,
1097-0223
Language:
English
Publisher:
Wiley
Publication Date:
2013
detail.hit.zdb_id:
1491217-X
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