In:
Journal of General Virology, Microbiology Society, Vol. 83, No. 11 ( 2002-11-01), p. 2693-2697
Abstract:
Infectious salmon anaemia virus (ISAV) infects cells via the endocytic pathway and, like many other enveloped viruses, ISAV contains a receptor-destroying enzyme. We have analysed this acetylesterase activity with respect to substrate specificity, enzyme kinetics, inhibitors, temperature and pH stability. The ISAV acetylesterase was inhibited by di-isopropyl fluorophosphate (DFP) in a dose-dependent fashion but not by other known hydrolase inhibitors, suggesting that a serine residue is part of the active site. The pH optimum of the enzyme was in the range 7·5–8·0 and the enzymatic activity was lessened at temperatures above 40 °C. The effect of DFP on agglutination/elution of erythrocytes by ISAV demonstrated that the acetylesterase activity is the bona fide receptor-destroying enzyme. A haemadsorption assay was used to analyse whether the esterase was active on the surface of infected cells or not.
Type of Medium:
Online Resource
ISSN:
0022-1317
,
1465-2099
DOI:
10.1099/0022-1317-83-11-2693
Language:
English
Publisher:
Microbiology Society
Publication Date:
2002
detail.hit.zdb_id:
2007065-2
SSG:
12
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