In:
Journal of the American Society for Horticultural Science, American Society for Horticultural Science, Vol. 124, No. 6 ( 1999-11), p. 644-648
Abstract:
Resistance to bean common mosaic virus (BCMV) strain NY15 (Zaumeyer) and bean common mosaic necrosis virus (BCMNV) strain NL-3 (Drijfhout) was assessed in 98 F 5:6 recombinant inbred lines (RILs) derived from a cross between pinto bean ( Phaseolus vulgaris L.) cultivars `Olathe' and `Sierra'. `Olathe' has bc-u and bc-1 2 whereas `Sierra' has no known resistance genes. The differentiation of resistant and susceptible lines was based on visual symptoms, virus titer, and top dry weight. Forty-seven RILs were moderately resistant to NL-3, while 51 RILs were susceptible. This segregation fits a 1 susceptible: 1 resistant ratio characteristic of a single gene. Sixty-nine RILs were susceptible to NY15, while 29 RILs were resistant, which fits a 3 susceptible: 1 resistant ratio characteristic of a two-gene model. Moderate resistance to NL-3 was conferred by bc-1 2 with or without bc-u present. Bulked segregant and two point linkage analysis identified randomly amplified polymorphic DNA (RAPD) markers linked in coupling to the Bc-1 and Bc-u alleles. The OH14 1100 RAPD marker was 4.5 cM from the Bc-1 locus. The OC16 1000 RAPD marker was linked at a distance of 10.9 cM from the Bc-u locus. Multipoint analysis, using segregation data for bc-1 2 , bc-u , and the two markers, estimated the distance between the Bc-1 and Bc-u loci as 22.8 cM.
Type of Medium:
Online Resource
ISSN:
0003-1062
,
2327-9788
DOI:
10.21273/JASHS.124.6.644
Language:
Unknown
Publisher:
American Society for Horticultural Science
Publication Date:
1999
detail.hit.zdb_id:
2040057-3
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