In:
Photochemistry and Photobiology, Wiley, Vol. 99, No. 1 ( 2023-01), p. 68-77
Abstract:
A new, simple‐to‐synthesize and sensitive turn‐on fluorogenic substrate ( CFMU‐Glu ) for β‐glucosidase activity was developed. This probe was based on a 7‐hydroxycoumarin derivative ( CFMU ) that could emit green fluorescence and had the low p K a value of 5.61 ± 0.01. CFMU‐Glu could be used for sensitive monitoring of the almond βGLU and Enterococcus faecalis ( E. faecalis ) at the optimal pHs of 6.50 and 7.00, respectively. Moreover, a new sensitive and selective fluorogenic broth (PBF‐B) for E. faecalis , utilizing CFMU‐Glu and polymyxin B, was also developed. Polymyxin B was discovered to can significantly improve the detection selectivity and signal intensity. The proposed 4‐four method using PBF‐B and a microcentrifuge tube could provide fluorogenic detection limits of 5.01 × 10 4 and 1.0 × 10 5 CFU mL −1 by fluorescence microplate reader and naked eye, respectively; it could also provide a turn‐on chromogenic detection limit of 1.0 × 10 6 CFU mL −1 by naked eye. The proposed method could detect 8 CFU mL −1 of E. faecalis in drinking water, Liangcha (herbal tea) and milk samples within 10 h, without pre‐enrichment.
Type of Medium:
Online Resource
ISSN:
0031-8655
,
1751-1097
Language:
English
Publisher:
Wiley
Publication Date:
2023
detail.hit.zdb_id:
2048860-9
SSG:
12
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