In:
Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 97, No. 3 ( 2000-02), p. 1062-1067
Abstract:
A comparative analysis of all published complete genomes indicated
that the putative orthologs of the unannotated ychB gene
of Escherichia coli follow the distribution of the dxs , dxr , and ygbP genes,
which have been shown to specify enzymes of the deoxyxylulose phosphate pathway of terpenoid biosynthesis, thus suggesting that the
hypothetical YchB protein also is involved in that pathway. To test this hypothesis, the E. coli ychB gene
was expressed in a homologous host. The recombinant protein was purified to homogeneity and was shown to phosphorylate
4-diphosphocytidyl-2C-methyl- d -erythritol in an
ATP-dependent reaction. The reaction product was identified as 4-diphosphocytidyl-2C-methyl- d -erythritol 2-phosphate by
NMR experiments with various 13 C-labeled substrate samples.
A 14 C-labeled specimen of this compound was converted
efficiently into carotenoids by isolated chromoplasts of Capsicum annuum . The sequence of E. coli YchB protein is similar to that of the protein predicted by the tomato
cDNA pTOM41 (30% identity), which had been implicated in the conversion of chloroplasts to chromoplasts.
Type of Medium:
Online Resource
ISSN:
0027-8424
,
1091-6490
DOI:
10.1073/pnas.97.3.1062
Language:
English
Publisher:
Proceedings of the National Academy of Sciences
Publication Date:
2000
detail.hit.zdb_id:
209104-5
detail.hit.zdb_id:
1461794-8
SSG:
11
SSG:
12
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