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  • 1
    Online Resource
    Online Resource
    Research Institute of Microsurgery ; 2023
    In:  Issues of Reconstructive and Plastic Surgery Vol. 25, No. 4 ( 2023-02-03), p. 53-64
    In: Issues of Reconstructive and Plastic Surgery, Research Institute of Microsurgery, Vol. 25, No. 4 ( 2023-02-03), p. 53-64
    Abstract: Objective: the purpose of the study was to evaluate the effects of three forms of allogeneic platelet concentrates on segmental tubular bone defect osseous regeneration. Material and Methods . In 145 laboratory rats tibial shaft segment was resected and stable osteosynthesis of the bone ends was performed. In group 1 animals the bone defect was left graftless, in group 2 animals biomaterial Collapan and shattered orthotopic autologous bone mixture into the bone defect site was placed. In group 3 animals as part of Collapan-autobone mixture defect filling fresh allogeneic platelet rich plasma was added, in group 4 animals allogeneic cryodestructed platelet rich plasma to the Collapan-bone mixture was added and group 5 animals received Collapan-bone mixture with allogeneic platelet rich fibrin added. Results. According to gross dissection data in 23 of the 32 group 3 rats, in 25 of the 37 group 4 rats and in 19 of the 27 group 5 rats complete visual tibial shaft defect healing occurred. In group 2 on visual inspection of tibial bone in 5 of the 10 rats defect healing was noted and in group 1 rats the defect healed in 15 of the 39 animals only. Bone defect healing rates in groups 3, 4 and 5 differed from group 1 healing rate significantly with χ 2 = 7,889, χ 2 = 6,452 and χ 2 = 6,504 respectively, group 2 bone defect healing rate did not differ from that of group 1 (χ 2 = 0,439). Histological analysis of specimens from groups 3, 5 and especially from group 4, at 3 weeks after plasty demonstrated active intermediary callus formation at bonesaw-line and neoformed osteoid bone on Collapan-bone chips aggregations surfaces; no intermediary callus formation in specimens from group 2 was found. At 6 to 12 weeks after plasty the most mature osseous tissue in specimens from group 4 was observed, the least mature neoformed osseous tissue amount was common to specimens from group 2. Conclusion. All three allogeneic Collapan-immobilized platelet concentrates studied promoted bone formation and healing of considerable segmental tibial shaft bone defect in rat model. The most evident osteogenesis activating effect was histologically confirmed in cases where cryodestructed platelet rich plasma to Collapan-bone chips mixture was added.
    Type of Medium: Online Resource
    ISSN: 1814-1471
    Language: Unknown
    Publisher: Research Institute of Microsurgery
    Publication Date: 2023
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  • 2
    In: Russian Sklifosovsky Journal "Emergency Medical Care", The Scientific and Practical Society of Emergency Medicine Physicians, Vol. 10, No. 1 ( 2021-04-20), p. 83-90
    Abstract: BACKGROUND Fractures of the proximal humerus are more common in older adults. Two options for surgical treatment of the same fractures are routinely performed — primary arthroplasty and osteosynthesis with metal fixators. Both methods are most effective in elderly patients for augmentation of the proximal humerus. For this purpose, methyl methacrylate cement or bone allo-/autografts are used. However, the incidence of unsatisfactory functional results after surgery remains high. AIM OF STUDY To develop allogeneic graft to strengthen the proximal humerus in the osteosynthesis of a comminuted fracture with a lack of bone tissue which has osteoconductive properties and capable of being a carrier of biologically active substances. MATERIAl AND METHODS The head, neck and portion of diaphysis of a fibula of a cadaver were used for a graft. The graft 6–8 cm long was formed and saturated with a collagen solution. Ten patients with 4-fragment fractures of the proximal humerus underwent surgery. X-rays and computed tomography of the operated joint were performed the day after surgery, and then, 6 weeks, 6 months, and one year after the surgery. The follow-up period ranged from 6 to 18 months. RESUlTS When evaluating osteoconductive effect in cell culture during saturation with collagen, the content cells on the surface of bone was 5–8 thousand/cm2 and 16–18 thousand/cm2 on the sample, saturated with plasma and growth factors. The studied bone grafts were biocompatible and non-toxic for fibroblast culture. On the surface of the bone graft without collagen adhesion of minimum number of cells occurred. After saturation of the graft with collagen, the adhesive activity of cells on the bone matrix increased, which indicated the presence of an osteoconductive effect. The addition of plasma saturated with growth factors promoted an increase in the number of adhered cells; therefore, the graft can be used as a carrier of biologically active substances. Postoperative X-ray didn’t reveal secondary varus displacement of the head in any patient (except for ununiformed fracture case). CONClUSION The developed graft is non-toxic, does not possess immunogenicity, has more pronounced osteoconductive properties in comparison with native bone allografts, which contributes to its colonization with cells. The use of such a graft in clinical practice makes it possible to achieve healing of the fracture and prevent secondary displacement of fragments. 
    Type of Medium: Online Resource
    ISSN: 2541-8017 , 2223-9022
    URL: Issue
    Language: Unknown
    Publisher: The Scientific and Practical Society of Emergency Medicine Physicians
    Publication Date: 2021
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  • 3
    Online Resource
    Online Resource
    The Scientific and Practical Society of Emergency Medicine Physicians ; 2022
    In:  Russian Sklifosovsky Journal "Emergency Medical Care" Vol. 11, No. 1 ( 2022-04-09), p. 199-209
    In: Russian Sklifosovsky Journal "Emergency Medical Care", The Scientific and Practical Society of Emergency Medicine Physicians, Vol. 11, No. 1 ( 2022-04-09), p. 199-209
    Abstract: The purpose of this work was to study the history of the development of traumatology-orthopedics at the N.V. Sklifosovsky Research Institute for Emergency Medicine. Traumatology as an independent discipline began to form at the N.V. Sklifosovsky Research Institute for Emergency Medicine during the reign of Professor S.S. Yudin (chief surgeon since 1928), who invited the famous Argentinean traumatologist-orthopedist Professor Lelio Zeno to work at the institute. In 1932, the trauma department of the N.V. Sklifosovsky Research Institute for Emergency Medicine was headed by Professor V.V. Gorinevskaya. She organized a 100-bed trauma clinic for the treatment of injuries to the head, spine, limbs, chest and abdominal cavity. She is rightfully considered one of the founders of trauma science in the USSR. With the development of traumatology and orthopedics as an independent specialty in 1961, two clinics were formed at the institute. The first trauma clinic was run by Dr. med. sciences professor I.I. Sokolov. The second clinic was headed by Dr. med. Sci. P.N. Petrov. In 1971, Dr. med. Professor, Honored Scientist of the Russian Federation, Academician of the Russian Academy of Medical and Technical Sciences of the Russian Federation V.P. Okhotsky was appointed the head of the traumatological service of the institute and the chief traumatologist of Moscow (from 1971 to 2001). With his active participation in Moscow, the reorganization of outpatient care was completed, work was widely launched on the medical aspects of the prevention of road traffic injuries and the introduction into practice of the most rational methods of diagnosis and treatment of patients with injuries of the musculoskeletal system. In the N.V. Sklifosovsky Research Institute for Emergency Medicine A.G. Suvalyan introduced the method of intramedullary osteosynthesis of long tubular bones (humerus, femur and tibia). Under the guidance of prof. V.P. Okhotsky the dissertation researches of A.G. Suvalyan, M.A. Suvalyan and S.S. Myakota were carried out. The expediency of early surgical intervention on the extremities in case of combined traumatic brain injury and multiple trauma of the extremities has also been proven, new functional methods of treatment of diaphyseal fractures of the shoulder, lower leg, spine and intra-articular injuries have been developed. In close scientific cooperation with scientists from other clinics of the institute, a comprehensive method of treating open injuries of the limbs has been developed (dissertation researches of I.F. Byalik, I.Yu. Klyukvin, O.P. Filippov, M.V. Zvezdina, R.S. Titov). Much scientific work has been carried out to improve the diagnosis and treatment of intra-articular injuries of the knee joint (dissertation researches by M.A. Malygina, O.P. Filippov, A.Yu. Vasa). From 2001 to 2016 the head of the department of emergency traumatology of the musculoskeletal system was a student of prof. V.P. Okhotsky – Dr. med. sciences, Professor, Academician of the Academy of Medical and Technical Sciences of the Russian Federation I.Yu. Klyukvin. Since 2016 the department is headed by Dr. med. sciences Alexey M. Fine. The scientific developments of the employees of the Department of Emergency Traumatology have a practical orientation. Every year, the department’s employees successfully perform more than 2500 surgical interventions using advanced low-traumatic techniques, making extensive use of the achievements of biotechnology.
    Type of Medium: Online Resource
    ISSN: 2541-8017 , 2223-9022
    Language: Unknown
    Publisher: The Scientific and Practical Society of Emergency Medicine Physicians
    Publication Date: 2022
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  • 4
    Online Resource
    Online Resource
    The Scientific and Practical Society of Emergency Medicine Physicians ; 2023
    In:  Russian Sklifosovsky Journal "Emergency Medical Care" Vol. 11, No. 4 ( 2023-02-03), p. 655-667
    In: Russian Sklifosovsky Journal "Emergency Medical Care", The Scientific and Practical Society of Emergency Medicine Physicians, Vol. 11, No. 4 ( 2023-02-03), p. 655-667
    Abstract: RELEVANCE The overall incidence of Achilles tendon rupture has been increasing in recent decades due to population aging, the growing prevalence of obesity and increased participation in sports. Achilles tendon ruptures are common injuries of the musculoskeletal system, and according to various authors, they account for 47% of all ruptures of human tendons and muscles, about 18 cases per 100 thousand people per year. Despite the abundance of various options of tendon suture in surgery, a wide choice of suture material and the use of precision techniques in tendon reconstruction, the proposed methods of macroscopic reconstruction of the tendon apparatus do not solve the problem of tendon suture failure. CONCLUSION Despite the abundance of proposed accesses to the Achilles tendon, the optimal one for all types of injuries has not yet been developed. Currently, there are no clear recommendations for choosing a specific method of treating an Achilles tendon rupture.
    Type of Medium: Online Resource
    ISSN: 2541-8017 , 2223-9022
    Language: Unknown
    Publisher: The Scientific and Practical Society of Emergency Medicine Physicians
    Publication Date: 2023
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  • 5
    In: Proceedings of the National Academy of Sciences, Proceedings of the National Academy of Sciences, Vol. 106, No. 25 ( 2009-06-23), p. 10272-10277
    Abstract: Adequate responses by our innate immune system toward invading pathogens were of vital importance for surviving infections, especially before the antibiotic era. Recently, a polymorphism in Mal (Ser180Leu, TIRAP rs8177374), an important adaptor protein downstream of the Toll-like receptor (TLR) 2 and 4 pathways, has been described to provide protection against a broad range of infectious pathogens. We assessed the functional effects of this polymorphism in human experimental endotoxemia, and we demonstrate that individuals bearing the TIRAP 180L allele display an increased, innate immune response to TLR4 and TLR2 ligands, but not to TLR9 stimulation. This phenotype has been related to an increased resistance to infection. However, an overshoot in the release of proinflammatory cytokines by TIRAP 180L homozygous individuals suggests a scenario of balanced evolution. We have also investigated the worldwide distribution of the Ser180Leu polymorphism in 14 populations around the globe to correlate the genetic makeup of TIRAP with the local infectious pressures. Based on the immunological, clinical, and genetic data, we propose that this mutation might have been selected in West Eurasia during the early settlement of this region after the out-of-Africa migration of modern Homo sapiens . This combination of functional and genetic data provides unique insights to our understanding of the pathogenesis of sepsis.
    Type of Medium: Online Resource
    ISSN: 0027-8424 , 1091-6490
    RVK:
    RVK:
    Language: English
    Publisher: Proceedings of the National Academy of Sciences
    Publication Date: 2009
    detail.hit.zdb_id: 209104-5
    detail.hit.zdb_id: 1461794-8
    SSG: 11
    SSG: 12
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  • 6
    In: Traumatology and Orthopedics of Russia, ECO-Vector LLC, Vol. 26, No. 2 ( 2020-07-09), p. 128-138
    Abstract: The study purpose — to evaluate the clinical results and the condition of bone tunnels after anterior cruciate ligament reconstruction with a semitendinosus tendon graft using cortical fixation and corrugated sutures.Materials and Methods. The results anterior cruciate ligament reconstruction with a semitendinosus tendon autograft were analyzed in 57 patients aged 18 to 53 years. The patients of the first group (n = 27) underwent anterior cruciate ligament reconstruction with a semitendinosus tendon graft using cortical fixation on the femur and tibia in combination with corrugated sutures at the proximal and distal ends of the graft. The patients of the second group (n = 30) underwent anterior cruciate ligament reconstruction in a similar way, but without the use of corrugated sutures. Clinical results were assessed using the Lysholm and IKDC scales. The degree of bone tunnels widening was evaluated by CT data in 6 months after the surgery.Results. In the first group, the degree of postoperative bone tunnels widening was significantly lower (for the femoral tunnel by 18% and tibial — by 17%) compared with the second group (for the femoral tunnel by 30% and tibial — by 31%). Scores by the IKDC 2000 and Lysholm scales were higher in the corrugated sutured group. Although, the treatment outcome was interpreted as equally good for both groups. The time for graft preparation was on average 6 minutes longer in the first group. This slightly increased the duration of the surgery.Conclusion. The anterior cruciate ligament reconstruction with a semitendinosus tendon graft using cortical fixation on the femur and tibia in combination with corrugated sutures ensured the tight contact of the tendon inside the bone tunnels without additional implants and reduced the degree of tunnels widening. This is important for a possible re-grafting. The proposed method does not significantly affect the clinical outcomes.
    Type of Medium: Online Resource
    ISSN: 2542-0933 , 2311-2905
    URL: Issue
    Language: Unknown
    Publisher: ECO-Vector LLC
    Publication Date: 2020
    detail.hit.zdb_id: 3070168-5
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  • 7
    In: Transplantologiya. The Russian Journal of Transplantation, IPO Association of Transplantologists, Vol. 14, No. 3 ( 2022-09-17), p. 344-356
    Abstract: The previous two articles described the use of platelet-rich plasma and platelet lysate. This part of the literature review examines the mechanism of red bone marrow action, indications and contraindications for its use. The results of treatment for delayed consolidation of bone fractures are also described. Hematopoietic stem cells give rise to all cellular components of the circulating blood, such as red blood cells, lymphocytes, neutrophils, and platelets. The most rational way to stimulate bone regeneration is to use the patient's own biological material. The aim of this article is to summarize the results of treatment using autologus bone marrow to improve bone regenerative potential in orthopaedics.
    Type of Medium: Online Resource
    ISSN: 2542-0909 , 2074-0506
    URL: Issue
    Language: Unknown
    Publisher: IPO Association of Transplantologists
    Publication Date: 2022
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  • 8
    Online Resource
    Online Resource
    The Scientific and Practical Society of Emergency Medicine Physicians ; 2017
    In:  Sklifosovsky Journal "Emergency Medical Care" Vol. 6, No. 3 ( 2017-01-01), p. 280-286
    In: Sklifosovsky Journal "Emergency Medical Care", The Scientific and Practical Society of Emergency Medicine Physicians, Vol. 6, No. 3 ( 2017-01-01), p. 280-286
    Type of Medium: Online Resource
    ISSN: 2541-8017 , 2223-9022
    URL: Issue
    Language: Unknown
    Publisher: The Scientific and Practical Society of Emergency Medicine Physicians
    Publication Date: 2017
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  • 9
    In: Genes & Immunity, Springer Science and Business Media LLC, Vol. 7, No. 7 ( 2006-10-01), p. 550-554
    Type of Medium: Online Resource
    ISSN: 1466-4879 , 1476-5470
    Language: English
    Publisher: Springer Science and Business Media LLC
    Publication Date: 2006
    detail.hit.zdb_id: 2006268-0
    SSG: 12
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  • 10
    In: Practical medicine, LLC Praktika, Vol. 20, No. 4 ( 2022), p. 117-122
    Abstract: The paper is dedicated to improving the treatment outcomes and consequences in elderly patients with femoral neck fracture by improving the diagnostics, osteosynthesis and total hip arthroplasty. The main causes and factors influencing the complication rates and final treatment outcomes in elderly patients with femoral neck fracture were identified. The evidence based diagnostic and treatment algorithm for such patients was designed. The proposed treatment system helped to reduce lethality, general and local complication rates among these patients. The active surgery helps to solve massive healthcare problems by easing the pain in elderly patients, restoring the hip joint mobility and weight bearing ability of extremity, enhancing independent living skills, and improving vitality and psychological state.
    Type of Medium: Online Resource
    ISSN: 2072-1757 , 2307-3217
    Uniform Title: Особенности хирургической тактики в лечении переломов проксимального отдела бедренной кости у геронтологических пациентов
    Language: Unknown
    Publisher: LLC Praktika
    Publication Date: 2022
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