In:
Critical Care Medicine, Ovid Technologies (Wolters Kluwer Health), Vol. 49, No. 9 ( 2021-09), p. 1513-1523
Abstract:
There is an unmet need to improve the description of the state of T-cell exhaustion in patients with sepsis, its reproducibility and correlation with the outcomes before including immunotherapy (like recombinant interleukin-7 or immune checkpoint inhibitors) in the therapeutic armamentarium against sepsis. DESIGN: Observational prospective study. SETTING: Two ICUs in a teaching hospital (France). PATIENTS: Eighty patients with sepsis admitted to the ICU. INTERVENTIONS: Quantification of CD4 + and CD8 + T-cell exhaustion at days 1 and 3. Quantification of the exhaustion markers (programmed death [PD]-1, 2B4, and cluster of differentiation [CD] 160) on T cells, the number of CD4 + regulatory T cells (CD3 + CD4 + CD25 hi CD127Lo cells), and the phorbol myristate acetate/ionomycin/ionomycin-induced cytokines production (tumor necrosis factor-α, interleukin-2, and interferon-γ). MEASUREMENTS AND MAIN RESULTS: Using unsupervised clustering analysis, patients could be split in three clusters according to their dominant pattern expression of exhaustion markers on CD8 + T cells (i.e., 2B4 low PD-1 low CD160 low , 2B4 hi PD-1 hi CD160 low , and 2B4 hi PD-1 low CD160 hi ) regardless of their underlying morbidities. Only 2B4 hi PD-1 hi CD160 low CD8+ T cells had cytokine production defect, whereas 2B4 hi PD-1 low CD160 hi pattern correlated with cytokine overproduction. Patients with a predominant “highly activated” 2B4 hi PD-1 low CD160 hi pattern did not develop secondary bacterial infections. By multivariate analysis, Simplified Acute Physiology Score 2 gravity score at day 1 ( p = 0.003) and patterns of exhaustion markers on CD8 + T cells ( p = 0.03) were associated with the risk of death. Neither the level of CD4 + regulatory T cells nor the CD4 + exhaustion patterns were associated with the outcomes. CONCLUSIONS: Easy-to-use multicolor flow cytometry assessing 2B4, PD-1, and CD160 expression on CD8 + T cells at day 1 identifies septic patients with poor outcome and discriminates patient subsets in who immunomodulatory drugs should be tested.
Type of Medium:
Online Resource
ISSN:
0090-3493
DOI:
10.1097/CCM.0000000000005047
Language:
English
Publisher:
Ovid Technologies (Wolters Kluwer Health)
Publication Date:
2021
detail.hit.zdb_id:
2034247-0
Permalink